The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast

Elife. 2019 Dec 19;8:e49784. doi: 10.7554/eLife.49784.


Previously we reported that a process called inter-fork strand annealing (IFSA) causes genomic deletions during the termination of DNA replication when an active replication fork converges on a collapsed fork (Morrow et al., 2017). We also identified the FANCM-related DNA helicase Fml1 as a potential suppressor of IFSA. Here, we confirm that Fml1 does indeed suppress IFSA, and show that this function depends on its catalytic activity and ability to interact with Mhf1-Mhf2 via its C-terminal domain. Finally, a plausible mechanism of IFSA suppression is demonstrated by the finding that Fml1 can catalyse regressed fork restoration in vitro.

Keywords: DNA replication; Rad52; S. pombe; chromosomes; gene expression; homologous recombination; replication fork barrier; replication fork collapse; replication termination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosomal Proteins, Non-Histone / genetics*
  • DNA Helicases / genetics*
  • DNA Replication / genetics
  • Genome, Fungal / genetics
  • Mitosis / genetics
  • Recombination, Genetic*
  • Schizosaccharomyces / genetics
  • Schizosaccharomyces pombe Proteins / genetics*


  • Chromosomal Proteins, Non-Histone
  • Mhf2 protein, S pombe
  • Schizosaccharomyces pombe Proteins
  • Fml1 protein, S pombe
  • DNA Helicases
  • Mhf1 protein, S pombe