Quantitative analysis of the distribution of serotonin-immunoreactive cell bodies in the mouse brain

Neurosci Lett. 1988 Sep 12;91(3):265-70. doi: 10.1016/0304-3940(88)90691-x.


The distribution of serotonin neurons in the mouse brain was studied using a peroxidase-antiperoxidase (PAP) immunohistochemical method to obtain quantitative data on serotonin-containing cell bodies. The average total number of perikarya of serotonin neurons was about 26,000, of which about 17,000 cells (66.9%) were situated in the raphe system. The nucleus raphe dorsalis was the main region where a large number of serotonin neurons were present, and it contained about 9000 cells. Other than the raphe system, serotonin-immunoreactive perikarya were seen in the following regions: the reticular formation (18.9%), the lemniscus medialis (8.5%), the nucleus interpeduncularis (1.5%), the subpyramidal region (4.0%) and the periaqueductal gray (0.2%).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / cytology*
  • Brain / metabolism
  • Cell Count
  • Immunohistochemistry
  • Male
  • Mice
  • Mice, Inbred ICR
  • Neurons / classification
  • Neurons / metabolism*
  • Serotonin / metabolism*


  • Serotonin