The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents. The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-TOC) and l-tryptophan composite (Trp-TOC) respectively. The antimicrobial studies were carried out on (i) Gram-negative bacteria: Escherichia coli (NCTC-10416) and Pseudomonas aeruginosa (NCID-9016); (ii) Gram-positive bacteria: Streptococcus aurous (NCTC-7447) and Bacillus subtilis (NCID-3610); (iii) unicellular fungi: namely, Candida albicans (NCCLS 11). The results were cleared that the both composites have high effective, rapid and broad-spectrum antimicrobial activity. The Trp-TOC showed slightly higher antimicrobial activity than Phe-TOC especially in time required of killing performance. The Phe-TOC has required 20 h for killing all microbial population while Trp-TOC required only 12 h. The MIC values were close in both nanocomposites with high clear zone measurements in the same concentration in the case of Trp-TOC.
Keywords: Amino acids; Antimicrobial; Cellulose derivative; Nanocomposites.
Copyright © 2019 Elsevier B.V. All rights reserved.