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, 14 (1), 20-23

Cloning and Analysis of PRNP Gene of Vulpes corsac in Qinghai Plateau, China

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Cloning and Analysis of PRNP Gene of Vulpes corsac in Qinghai Plateau, China

Xue-Hua Yang et al. Prion.

Abstract

PRNP gene encodes PrP protein, which is conservative among different species and associates with the susceptibility of prion disease. In this report, we cloned and sequenced the full-length PRNP gene of Vulpes corsac in Qinghai plateau, China. The amino acid sequence of Vulpes corsac PrP showed 100% homology with those of the other three species of foxes. The taxa relationship of Vulpes corsac PrP with other species of animals, including human, canine, bovine, cervus, capra, ovis, camelus, felis, Mustela, mouse and hamster were also analysed.

Keywords: PRNP; Qinghai plateau; Vulpes corsac; prion disease.

Figures

Figure 1.
Figure 1.
Homology analysis of amino acid sequences of PrP proteins of human and various species of animals. (a). Phylogenetic tree. (b). Homology matrix.
Figure 2.
Figure 2.
Comparison of the variations of amino acids of PrP proteins of human and various animals. The various functional and secondary structural regions within PrP sequences are indicated with colours. The amino acid numbers are shown on the right.

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Grant support

This work was supported by Research Project [2018ZX10711001, 2017YFC1200500], Chinese National Natural Science Foundation Grants [81572048, 81630062] and SKLID Development Grant [2019SKLID501, 2019SKLID603, 2019SKLID307].
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