Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA

Methods Mol Biol. 2020:2099:127-133. doi: 10.1007/978-1-0716-0211-9_11.

Abstract

Indirect enzyme-linked immunosorbent assay (ELISA) enables detection and quantification of antigen-specific antibodies in biological samples such as human or animal sera. Most current MERS-CoV serological assays such as neutralization, immunofluorescence, or protein microarray rely on handling of live MERS-CoV in high containment laboratories, highly trained personnel as well as the need for expensive and special equipment and reagents representing a hurdle for most laboratories especially when resources are limited. In this chapter, we describe a validated and optimized indirect ELISA protocol based on recombinant S1 subunit (amino acids 1-725) of MERS-CoV for qualitative and quantitative determination of MERS-CoV-binding antibodies.

Keywords: Antibodies; Antigens; ELISA; MERS-CoV; Recombinant S1 subunit; Serology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Neutralizing / immunology*
  • Antibodies, Viral / immunology
  • Coronavirus Infections / virology*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Humans
  • Middle East Respiratory Syndrome Coronavirus / immunology*
  • Middle East Respiratory Syndrome Coronavirus / isolation & purification
  • Recombinant Proteins
  • Spike Glycoprotein, Coronavirus / immunology*

Substances

  • Antibodies, Monoclonal
  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Recombinant Proteins
  • Spike Glycoprotein, Coronavirus