Autophagy Induction by HDAC Inhibitors Is Unlikely to be the Mechanism of Efficacy in Prevention of Retinal Degeneration Caused by P23H Rhodopsin

Runxia H Wen; Aaron D Loewen; Ruanne Y J Vent-Schmidt; Orson L Moritz

doi:10.1007/978-3-030-27378-1_66

Autophagy Induction by HDAC Inhibitors Is Unlikely to be the Mechanism of Efficacy in Prevention of Retinal Degeneration Caused by P23H Rhodopsin

Adv Exp Med Biol. 2019:1185:401-405. doi: 10.1007/978-3-030-27378-1_66.

Authors

Runxia H Wen¹, Aaron D Loewen¹, Ruanne Y J Vent-Schmidt¹, Orson L Moritz²

Affiliations

¹ Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, BC, Canada.
² Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, BC, Canada. olmoritz@mail.ubc.ca.

PMID: 31884645
DOI: 10.1007/978-3-030-27378-1_66

Abstract

We previously found that valproic acid (VPA) and other histone deacetylase inhibitors (HDACis) ameliorate retinal degeneration (RD) caused by P23H rhodopsin in Xenopus laevis larvae and hypothesized that this may be due to enhancement of autophagy. Here we use X. laevis expressing an autophagy marker to assess effects of HDACis on autophagy. We also assess the effects of non-HDACi activators and inducers of autophagy on RD caused by P23H rhodopsin.

Keywords: Autophagy; Chloroquine; Histone deacetylase inhibitor; Rapamycin; Retinitis pigmentosa; Transgenic Xenopus laevis; Valproic acid.

MeSH terms

Animals
Autophagy*
Disease Models, Animal
Histone Deacetylase Inhibitors / pharmacology*
Larva
Retinal Degeneration / chemically induced
Retinal Degeneration / prevention & control*
Rhodopsin / adverse effects*
Xenopus laevis

Substances

Histone Deacetylase Inhibitors
Rhodopsin