Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species

Andrew G Hettle; Joanne K Hobbs; Benjamin Pluvinage; Chelsea Vickers; Kento T Abe; Orly Salama-Alber; Bailey E McGuire; Jan-Hendrik Hehemann; Joseph P M Hui; Fabrice Berrue; Arjun Banskota; Junzeng Zhang; Eric M Bottos; Jonathan Van Hamme; Alisdair B Boraston

doi:10.1038/s42003-019-0721-y

Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species

Commun Biol. 2019 Dec 19:2:474. doi: 10.1038/s42003-019-0721-y. eCollection 2019.

Authors

Andrew G Hettle¹, Joanne K Hobbs¹, Benjamin Pluvinage¹, Chelsea Vickers^{1

2}, Kento T Abe^{1

3}, Orly Salama-Alber¹, Bailey E McGuire¹, Jan-Hendrik Hehemann^{1

4}, Joseph P M Hui⁵, Fabrice Berrue⁵, Arjun Banskota⁵, Junzeng Zhang⁵, Eric M Bottos⁶, Jonathan Van Hamme⁶, Alisdair B Boraston¹

Affiliations

¹ 1Department of Biochemistry and Microbiology, University of Victoria, PO Box 1700 STN CSC, Victoria, British Columbia V8W 2Y2 Canada.
² 4Present Address: School of Biological Sciences, Victoria University, PO Box 600, Wellington, 6012 New Zealand.
³ 5Present Address: Lunenfeld-Tanenbaum Research Institute, Sinai Health System, and Department of Molecular Genetics, University of Toronto, 600 University Ave, Rm 992, Toronto, ON M5G1X5 Canada.
⁴ 6Present Address: Marum and Max Planck Institute for Marine Microbiology, Celsiusstraße 1, 28359 Bremen, Germany.
⁵ 2Aquatic and Crop Resource Development Research Centre, National Research Council of Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1 Canada.
⁶ 3Department of Biological Sciences, Thompson Rivers University, 805 TRU Way, Kamloops, British Columbia V2C 0C8 Canada.

Abstract

Pseudoalteromonas is a globally distributed marine-associated genus that can be found in a broad range of aquatic environments, including in association with macroalgal surfaces where they may take advantage of these rich sources of polysaccharides. The metabolic systems that confer the ability to metabolize this abundant form of photosynthetically fixed carbon, however, are not yet fully understood. Through genomics, transcriptomics, microbiology, and specific structure-function studies of pathway components we address the capacity of newly isolated marine pseudoalteromonads to metabolize the red algal galactan carrageenan. The results reveal that the κ/ι-carrageenan specific polysaccharide utilization locus (CarPUL) enables isolates possessing this locus the ability to grow on this substrate. Biochemical and structural analysis of the enzymatic components of the CarPUL promoted the development of a detailed model of the κ/ι-carrageenan metabolic pathway deployed by pseudoalteromonads, thus furthering our understanding of how these microbes have adapted to a unique environmental niche.

Keywords: Carbohydrates; Enzymes; Glycobiology; Structural biology; X-ray crystallography.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Aquatic Organisms / metabolism*
Binding Sites
Carrageenan / chemistry
Carrageenan / metabolism*
Gene Order
Glycoside Hydrolases / chemistry
Glycoside Hydrolases / metabolism
Metabolic Networks and Pathways*
Models, Molecular
Open Reading Frames
Protein Binding
Pseudoalteromonas / genetics
Pseudoalteromonas / metabolism*
Structure-Activity Relationship

Substances

Carrageenan
Glycoside Hydrolases

Abstract

Publication types

MeSH terms

Substances

Grants and funding