Architecture of the chromatin remodeler RSC and insights into its nucleosome engagement

Elife. 2019 Dec 30;8:e54449. doi: 10.7554/eLife.54449.


Eukaryotic DNA is packaged into nucleosome arrays, which are repositioned by chromatin remodeling complexes to control DNA accessibility. The Saccharomyces cerevisiae RSC (Remodeling the Structure of Chromatin) complex, a member of the SWI/SNF chromatin remodeler family, plays critical roles in genome maintenance, transcription, and DNA repair. Here, we report cryo-electron microscopy (cryo-EM) and crosslinking mass spectrometry (CLMS) studies of yeast RSC complex and show that RSC is composed of a rigid tripartite core and two flexible lobes. The core structure is scaffolded by an asymmetric Rsc8 dimer and built with the evolutionarily conserved subunits Sfh1, Rsc6, Rsc9 and Sth1. The flexible ATPase lobe, composed of helicase subunit Sth1, Arp7, Arp9 and Rtt102, is anchored to this core by the N-terminus of Sth1. Our cryo-EM analysis of RSC bound to a nucleosome core particle shows that in addition to the expected nucleosome-Sth1 interactions, RSC engages histones and nucleosomal DNA through one arm of the core structure, composed of the Rsc8 SWIRM domains, Sfh1 and Npl6. Our findings provide structural insights into the conserved assembly process for all members of the SWI/SNF family of remodelers, and illustrate how RSC selects, engages, and remodels nucleosomes.

Keywords: S. cerevisiae; chromatin; chromatin remodeling; cryo-EM; molecular biophysics; structural biology; transcription.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins / chemistry
  • Cell Cycle Proteins / genetics
  • Chromatin Assembly and Disassembly / genetics*
  • Chromosomal Proteins, Non-Histone / chemistry*
  • Chromosomal Proteins, Non-Histone / genetics
  • Cryoelectron Microscopy
  • DNA Repair / genetics
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / ultrastructure
  • Multiprotein Complexes / chemistry
  • Multiprotein Complexes / genetics
  • Nucleosomes / chemistry*
  • Nucleosomes / genetics
  • Nucleosomes / ultrastructure
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / ultrastructure
  • Saccharomyces cerevisiae Proteins / chemistry*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / ultrastructure
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics
  • Transcription Factors / ultrastructure


  • Cell Cycle Proteins
  • Chromosomal Proteins, Non-Histone
  • DNA, Fungal
  • DNA-Binding Proteins
  • Multiprotein Complexes
  • NPL6 protein, S cerevisiae
  • Nucleosomes
  • RSC complex, S cerevisiae
  • SWI-SNF-B chromatin-remodeling complex
  • Saccharomyces cerevisiae Proteins
  • Sfh1 protein, S cerevisiae
  • Transcription Factors

Associated data

  • PDB/6V8O
  • PDB/6V92