Proteomics and metabolomics analysis of tomato fruit at different maturity stages and under salt treatment

Huimeng Tang; Xu Zhang; Biao Gong; Yanyan Yan; Qinghua Shi

doi:10.1016/j.foodchem.2019.126009

Proteomics and metabolomics analysis of tomato fruit at different maturity stages and under salt treatment

Food Chem. 2020 May 1:311:126009. doi: 10.1016/j.foodchem.2019.126009. Epub 2019 Dec 10.

Authors

Huimeng Tang¹, Xu Zhang¹, Biao Gong¹, Yanyan Yan¹, Qinghua Shi²

Affiliations

¹ State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 2018, PR China.
² State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 2018, PR China. Electronic address: qhshi@sdau.edu.cn.

PMID: 31887558
DOI: 10.1016/j.foodchem.2019.126009

Abstract

Proteomics and metabolomics were used to study the changes in proteins and metabolites in tomato fruits at different ripening stages and the effect of salt treatment on fruit quality. The results showed 2607 and 153 differentially expressed proteins in ripe fruits compared with mature green fruits and in NaCl-treated ripe fruits compared with control ripe fruits, respectively. KEGG analysis indicated that these proteins were mainly involved in photosynthesis, pentose and glucuronate interconversions in different ripening stages of fruits, and salt-induced proteins were involved in flavonoid biosynthesis and linoleic acid metabolism. A series of metabolites, including carbohydrates and amino acids showed significantly different accumulations between ripe and mature green fruits and between salt-treated and control fruits. Combined analysis explored glycine, L-alanine, D-xylose and sucrose and some proteins involved in multiple metabolic pathways under salt conditions. Their interactions might affect fruit development and fruit quality under salt treatment.

Keywords: Fruit ripening; Metabolites; Proteomics; Salt treatment; Tomato fruit quality.

MeSH terms

Amino Acids / metabolism
Fatty Acids / metabolism
Fruit / drug effects
Fruit / metabolism
Metabolic Networks and Pathways / drug effects*
Metabolomics*
Plant Proteins / metabolism
Proteomics*
Sodium Chloride / pharmacology*
Solanum lycopersicum / drug effects
Solanum lycopersicum / metabolism*

Substances

Amino Acids
Fatty Acids
Plant Proteins
Sodium Chloride