Let me infuse this for you - A way to solve the first YPIC challenge

EuPA Open Proteom. 2019 Aug 16;22-23:19-21. doi: 10.1016/j.euprot.2019.07.007. eCollection 2019 Mar.


In a common proteomics analysis today, the origins of our sample in the vial are known and therefore a database dependent approach to identify the containing peptides can be used. The first YPIC challenge though provided us with 19 synthetic peptides, which together formed an English sentence. For the identification of these peptides, a de-novo approach was used, which brought us together with an internet search engine to the hidden sentence. But only having the sentence was not sufficient for us, we also wanted to identify as many as possible of the spectra in our data. Therefore, we created and refined a database approach from the de-novo method and finally could identify the peptide-sentence with a good overlap.