Simultaneous determination of five metal ions by on-line complexion combined with micelle to solvent stacking in capillary electrophoresis

Xiaoyu Song; Rui Zhang; Yue Wang; Mengqing Feng; Honghua Zhang; Shuling Wang; Jun Cao; Tian Xie

doi:10.1016/j.talanta.2019.120578

Simultaneous determination of five metal ions by on-line complexion combined with micelle to solvent stacking in capillary electrophoresis

Talanta. 2020 Mar 1:209:120578. doi: 10.1016/j.talanta.2019.120578. Epub 2019 Nov 21.

Authors

Xiaoyu Song¹, Rui Zhang¹, Yue Wang¹, Mengqing Feng¹, Honghua Zhang¹, Shuling Wang², Jun Cao³, Tian Xie⁴

Affiliations

¹ Medical College, Hangzhou Normal University, Hangzhou, 311121, PR China.
² Medical College, Hangzhou Normal University, Hangzhou, 311121, PR China. Electronic address: wsling222@163.com.
³ Medical College, Hangzhou Normal University, Hangzhou, 311121, PR China; College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 311121, PR China. Electronic address: caoj@hznu.edu.cn.
⁴ Medical College, Hangzhou Normal University, Hangzhou, 311121, PR China; Holistic Integrative Pharmacy Institutes, Hangzhou Normal University, Hangzhou, 311121, PR China; Key Laboratory of Elemene Anti-cancer Medicine of Zhejiang Province, Hangzhou, 311121, PR China; Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang Province, Hangzhou, 311121, PR China. Electronic address: xbs@hznu.edu.cn.

PMID: 31892076
DOI: 10.1016/j.talanta.2019.120578

Abstract

A direct on-line complexion combined with micelle to solvent stacking method was proposed for simultaneous determination of metal ions by capillary electrophoresis coupled diode array detector. During the experiment, a plug of complexing agent was first injected to the inlet of capillary, followed by introducing the micelle-bound metal ions. Then the metal ions produced a micelle-to-solvent stacking effect and interacted with the complexing agent under a positive voltage. Continued application of voltage, the analytes were effectively focused and separated in the capillary zone electrophoresis. Repeatability was ranged from 1.89% to 1.94% for the migration time. The detection limits were 2.66-27.9 ng mL^-1 for Ni²⁺, Co²⁺, Cu²⁺, Hg²⁺ and Cd²⁺. Furthermore, the developed method showed a great potential for the determination of metal ions in the crayfish, beebread and Dendrobium officinale samples.

Keywords: Beebread; Capillary electrophoresis; Dendrobium officinale; Metal ions; Micelle to solvent stacking; On-line complexion.