Aim of the study: Assessment of hepatic expression of vascular endothelial growth factor A (VEGF-A) in liver tissues of infants with biliary atresia (BA).
Material and methods: This retrospective study included 35 infants with BA (BA group), and 38 infants with cholestasis due to causes other than BA (non-BA group). All patients had undergone full history taking, through clinical examination, routine investigations and immunostaining of liver tissue for VEGF-A and cytokeratin 7 (CK7). The diagnosis of BA was confirmed by intraoperative cholangiography. In the non-BA group, other specific laboratory tests according to the expected etiology were done.
Results: Most of the BA group showed positive VEGF-A expression with variable degrees in both bile ducts (BDs; 80%), and arterial walls (AWs; 77.2%), while most of the non-BA group showed negative staining of VEGF in both BDs and AWs (89.5% and 86.8% respectively) (p < 0.0001). Positive VEGF expression in the portal structures in both BDs and AWs had 84.9% and 82.19% accuracy; respectively. The majority of BA group showed either grade II of positive cytokeratin-7 expression in liver tissues (45.7%) or grade III (34.3%), while most of the non-BA group showed grade I (71.1%) (p < 0.0001). Positive CK7 expression in > 25% of the liver tissues had 80.8% accuracy in discriminating between BA and non-BA.
Conclusions: VEGF-A expression in the portal structures in liver tissues in both BDs and AWs had very good accuracy in discriminating between BA and non-BA patients.
Keywords: biliary atresia; cytokeratin; growth factor.
Copyright: © 2019 Clinical and Experimental Hepatology.
Conflict of interest statement
The authors report no conflict of interest.
Immunolocalization of VEGF A and Its Receptors, VEGFR1 and VEGFR2, in the Liver From Patients With Biliary AtresiaPT Edom et al. Appl Immunohistochem Mol Morphol 19 (4), 360-8. PMID 21285868.In biliary atresia (BA), a cholangiopathy of elusive etiology invariably leads to cirrhosis, and a disturbed angiogenesis may be involved. We evaluated the hepatobiliary …
Histopathological Expression of Yes-associated Protein in Neonatal CholestasisSS Abo-Zeinah et al. Clin Res Hepatol Gastroenterol. PMID 31279668.Hepatic expression of YAP was significantly higher in BA than in non-BA group and could discriminate BA from other causes of cholestasis.
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Differential Hepatic Expression of CD56 Can Discriminate Biliary Atresia From Other Neonatal Cholestatic DisordersMM Sira et al. Eur J Gastroenterol Hepatol 24 (10), 1227-33. PMID 22772093.CD56 immunostaining of the liver had a diagnostic value; it can be used to differentiate BA from other neonatal cholestatic disorders and might be useful as an additional …
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