Single-cell RNA sequencing of human kidney

Sci Data. 2020 Jan 2;7(1):4. doi: 10.1038/s41597-019-0351-8.


A comprehensive cellular anatomy of normal human kidney is crucial to address the cellular origins of renal disease and renal cancer. Some kidney diseases may be cell type-specific, especially renal tubular cells. To investigate the classification and transcriptomic information of the human kidney, we rapidly obtained a single-cell suspension of the kidney and conducted single-cell RNA sequencing (scRNA-seq). Here, we present the scRNA-seq data of 23,366 high-quality cells from the kidneys of three human donors. In this dataset, we show 10 clusters of normal human renal cells. Due to the high quality of single-cell transcriptomic information, proximal tubule (PT) cells were classified into three subtypes and collecting ducts cells into two subtypes. Collectively, our data provide a reliable reference for studies on renal cell biology and kidney disease.

Publication types

  • Dataset
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Humans
  • Kidney / cytology*
  • Kidney Tubules, Collecting / cytology
  • Kidney Tubules, Proximal / cytology
  • RNA-Seq*
  • Single-Cell Analysis*
  • Transcriptome