The spectrophotometric determination of nanomole quantities of chloride in plant tissue

Anal Biochem. 1988 Aug 15;173(1):54-8. doi: 10.1016/0003-2697(88)90158-3.


The determination of Cl- in plant tissues was investigated by FeSCN2+ spectrophotometry following SCN- displacement from Hg(SCN)2 by Cl-. The calibration curve was nonlinear, but consistent and reproducible. As little as 10 nmol Cl- could be measured. There was no obvious interference in color development by water-soluble components in plant tissues. Values obtained by this method were in close agreement with those obtained potentiometrically and by coulometric titration.

Publication types

  • Comparative Study

MeSH terms

  • Chlorides / analysis*
  • Ferrocyanides
  • Microchemistry
  • Plants / analysis*
  • Potentiometry
  • Solubility
  • Spectrophotometry / methods*
  • Thiocyanates
  • Water


  • Chlorides
  • Ferrocyanides
  • Thiocyanates
  • Water
  • ferrous thiocyanate