The effect of Liv-52 on liver ischemia reperfusion damage in rats

BMC Pharmacol Toxicol. 2020 Jan 3;21(1):2. doi: 10.1186/s40360-019-0380-0.

Abstract

Background: Liver ischemia reperfusion (I/R) damage which is frequently seen in clinical hepatobiliary surgeries has no effective treatment for it. Liv-52, known to have hepatoprotective effects, is a natural antioxidant drug licensed by the Ministry of Health of India. The aim of our study is to investigate the effect of Liv-52 on liver damage induced by I/R in rats.

Methods: Albino Wistar male rats were divided into three groups; liver I/R (IR), 20 mg/kg Liv-52 + liver ischemia reperfusion (LIR) and sham operation applied to control group (HG). Liv-52 was administered to the LIR group (n = 6) 1 h prior to I/R application and distilled water was given orally to IR (n = 6) and HG (n = 6) groups as a solvent. Ischemia was determined as 1 h, and reperfusion was identified as 6 h in animals.

Results: Increased levels of alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase, malondialdehyde, myeloperoxidase, and decreased levels of superoxide dismutase, and glutathione related enzymes caused by I/R application have been converged to healthy group level with Liv-52 treatment and the damage in liver tissue has been improved histopathologically.

Conclusions: Liv-52 may be beneficial for preventing liver I/R damage in pre-surgery application.

Keywords: Injury; Ischemia reperfusion; Liv-52; Liver; Rat.

MeSH terms

  • Alanine Transaminase / blood
  • Animals
  • Antioxidants / pharmacology
  • Antioxidants / therapeutic use*
  • Aspartate Aminotransferases / blood
  • Drug Combinations
  • Glutathione / metabolism
  • Glutathione Peroxidase / metabolism
  • Glutathione Reductase / metabolism
  • Glutathione Transferase / metabolism
  • L-Lactate Dehydrogenase / blood
  • Liver / drug effects*
  • Liver / metabolism
  • Liver / pathology
  • Male
  • Malondialdehyde / metabolism
  • Peroxidase / metabolism
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*
  • Rats, Wistar
  • Reperfusion Injury / blood
  • Reperfusion Injury / drug therapy*
  • Reperfusion Injury / metabolism
  • Reperfusion Injury / pathology
  • Superoxide Dismutase / metabolism

Substances

  • Antioxidants
  • Drug Combinations
  • Plant Extracts
  • Malondialdehyde
  • Liv 52
  • L-Lactate Dehydrogenase
  • Peroxidase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione Reductase
  • Glutathione Transferase
  • Aspartate Aminotransferases
  • Alanine Transaminase
  • Glutathione