The effect of oral hydrolyzed eggshell membrane on the appearance of hair, skin, and nails in healthy middle-aged adults: A randomized double-blind placebo-controlled clinical trial

J Cosmet Dermatol. 2020 Jun;19(6):1463-1472. doi: 10.1111/jocd.13275. Epub 2020 Jan 6.


Background: Many over the counter and consumer packaged goods are promoted to enhance the appearance of hair, skin, and nails for the consumer. Nutrition is a major factor in affecting the health and appearance of hair, skin, and nails. In addition to how one eats, dietary supplementation may play a role in overall health and in the physical appearance.

Aims: It was the aim of this study to objectively and subjectively evaluate the impacts of a nutritional intervention as compared to placebo on the appearances of hair, skin, and nails in healthy middle-aged adults.

Methods: Randomized, double-blind placebo-controlled study with 88 subjects randomized evenly to Study Product (BiovaBio™ 450 mg/d, n = 44) or Placebo (n = 44) for 12-weeks. Outcome tests included TrichoScan HD (hair), Canfield Visia® -CR (skin), modified FACE-Q (skin), and anchored Likert Scales (nails).

Results: Oral hydrolyzed eggshell membrane ingestion was associated with a significant improvement in facial skin appearance in crow's feet in 4 weeks and skin tone in 8 weeks, with significant impact on hair thickness, reduction in hair breakage and improvement in hair growth at 4, 8, and 12 weeks. There were no observed subjective improvements for nails (appearance, strength or growth).

Conclusions: Oral supplementation of 450 mg/d hydrolyzed eggshell membrane for 12 weeks is associated with improvement in the appearance of facial skin and hair.

Keywords: antiaging; eggshell membrane; glycosaminoglycan; hyaluronic acid; hydroxyproline.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Administration, Oral
  • Adult
  • Aged
  • Animals
  • Biological Products / administration & dosage*
  • Biological Products / adverse effects
  • Cohort Studies
  • Dietary Supplements / adverse effects*
  • Double-Blind Method
  • Egg Shell / chemistry*
  • Face
  • Female
  • Hair / drug effects*
  • Hair / growth & development
  • Humans
  • Male
  • Middle Aged
  • Nails / drug effects
  • Nails / growth & development
  • Placebos / administration & dosage
  • Placebos / adverse effects
  • Skin / drug effects*
  • Skin Aging / drug effects
  • Skin Pigmentation / drug effects
  • Treatment Outcome


  • Biological Products
  • Placebos