LncRNA TUG1 promoted osteogenic differentiation through promoting bFGF ubiquitination

In Vitro Cell Dev Biol Anim. 2020 Jan;56(1):42-48. doi: 10.1007/s11626-019-00410-y. Epub 2020 Jan 6.


LncRNA TUG1 has the potential to promote the osteogenic differentiation of several cells, but the role of lncRNA TUG1 in osteogenic differentiation of tendon stem/progenitor cells (TSPCs) is still unknown. This study aims to determine the role of lncRNA TUG1 in osteogenic differentiation of TSPCs. bFGF, RUNX2, and Osterix protein expressions were detected by western blot. LncRNA TUG1 and bFGF expression was detected by qRT-PCR. RNA immunoprecipitation (RIP) assay was used to confirm the interaction between TUG1 and bFGF2. Ubiquitination assay was used to determine the ubiquitination of bFGF protein. During osteogenic differentiation, the protein expression of bFGF was significantly downregulated in TSPCs, and the expression of TUG1 was significantly elevated in TSPCs. Interfering TUG1 or overexpressing bFGF suppressed osteogenic differentiation of TSPCs. In addition, lncRNA TUG1 interacted with bFGF, and lncRNA TUG1 promoted the ubiquitination of bFGF protein. We also determined that lncRNA TUG1 downregulated bFGF protein expression through promoting the ubiquitination of bFGF. LncRNA TUG1 promoted the osteogenic differentiation of TSPCs through promoting bFGF ubiquitination.

Keywords: LncRNA TUG1; Osteogenic differentiation; Tendon stem/progenitor cells; bFGF.

MeSH terms

  • Animals
  • Cell Differentiation / genetics*
  • Fibroblast Growth Factor 2 / metabolism*
  • Mice, Inbred C57BL
  • Osteogenesis / genetics*
  • Proteolysis
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / metabolism*
  • Stem Cells / metabolism
  • Tendons / cytology
  • Ubiquitination*


  • RNA, Long Noncoding
  • long non-coding RNA TUG1, mouse
  • Fibroblast Growth Factor 2