The effects of gilvocarcin V and ultraviolet radiation on pBR322 DNA and lymphocytes

Chem Biol Interact. 1988;67(3-4):255-65. doi: 10.1016/0009-2797(88)90062-2.

Abstract

The antitumor antibiotic gilvocarcin (GV) when photoactivated with UV radiation induced single strand breaks in superhelical pBR322 DNA. The optimal wavelengths for nicking DNA correlated with the absorbance maximum of GV near the visible region (398 nm). The response of lymphocytes to stimulation by phytohemagglutinin was reduced to 10% of controls at 0.10 ng/ml GV in combination with 3 J/cm2 of ultraviolet A (UVA) radiation. The potency of gilvocarcin is attributed to two factors: its strong tendency to intercalate with DNA (K = 6.6 X 10(5) M-1) and its intense absorption of UVA radiation (E398 = 11971 M-1 cm-1).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminoglycosides*
  • Anti-Bacterial Agents / pharmacology*
  • Coumarins
  • DNA / drug effects*
  • DNA / radiation effects*
  • DNA Damage*
  • DNA, Superhelical / drug effects
  • DNA, Superhelical / radiation effects
  • Glycosides
  • Humans
  • In Vitro Techniques
  • Intercalating Agents
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / radiation effects
  • Lymphocytes / drug effects
  • Lymphocytes / radiation effects
  • Methoxsalen / pharmacology
  • Photochemistry
  • Plasmids
  • Spectrum Analysis
  • Ultraviolet Rays

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents
  • Coumarins
  • DNA, Superhelical
  • Glycosides
  • Intercalating Agents
  • gilvocarcin V
  • DNA
  • Methoxsalen