Preparation of Restricted Access Media-Molecularly Imprinted Polymers for the Detection of Chloramphenicol in Bovine Serum

Shanwen Zhao; Chanling Wei; Zhian Sun; Huachun Liu; Yanqiang Zhou; Xiaoxiao Wang; Jianmin Li; Bolin Gong

doi:10.1155/2019/7930102

Preparation of Restricted Access Media-Molecularly Imprinted Polymers for the Detection of Chloramphenicol in Bovine Serum

J Anal Methods Chem. 2019 Dec 12:2019:7930102. doi: 10.1155/2019/7930102. eCollection 2019.

Authors

Shanwen Zhao¹, Chanling Wei^{1

2}, Zhian Sun¹, Huachun Liu¹, Yanqiang Zhou¹, Xiaoxiao Wang¹, Jianmin Li¹, Bolin Gong¹

Affiliations

¹ College of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, China.
² China Customs Ningxia Entry-Exit Inspection and Quarantine, Yinchuan 750000, China.

Abstract

Chloramphenicol- (CAP-) restricted access media-molecularly imprinted polymers (CAP-RAM-MIPs) were prepared by precipitation polymerization using CAP as a template molecule, 2-diethylaminoethyl methacrylate (DEAEM) as a functional monomer, ethylene glycol dimethyl acrylate (EDMA) as a crosslinking agent, glycidyl methacrylate (GMA) as an outer hydrophilic functional monomer, and acetonitrile as a pore former and solvent. The CAP-RAM-MIPs were successfully characterized by Fourier-transform infrared spectroscopy, scanning electron microscopy, and thermogravimetric analysis. The adsorption performance was investigated in detail using static, dynamic, and selective adsorption experiments. Adsorption equilibrium could be reached within 11 min. The CAP-RAM-MIPs had a high adsorption rate and good specific adsorption properties. Scatchard fitting curves indicated there were two binding sites for CAP-RAM-MIPs. Adsorption was Freundlich multilayer adsorption and consistent with the quasi-second kinetic model. Using CAP-RAM-MIPs for selective separation and enrichment CAP in bovine serum in combination with high-performance liquid chromatography (HPLC), CAP recovery ranged from 94.1 to 97.9% with relative standard deviations of 0.7-1.5%. This material has broad application prospects in enrichment and separation.