Differentiation of Rodentibacter pneumotropicus, Rodentibacter heylii and Muribacter muris by MALDI-TOF MS

J Microbiol Methods. 2020 Feb:169:105836. doi: 10.1016/j.mimet.2020.105836. Epub 2020 Jan 7.

Abstract

The pathogens Rodentibacter (R.) pneumotropicus and R. heylii as well as the commensal Muribacter (M.) muris are frequently isolated in mice. In this study, a MALDI-TOF MS database was extended with spectra of well characterized strains of these species. Compared to a multiplex PCR, all examined out-of-sample isolates were correctly identified.

Keywords: Colonization; Federation of European Laboratory Animal Science Associations (FELASA); Laboratory mice; Pasteurella pneumotropica; Rodentibacter ratti.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA, Bacterial / genetics
  • Mice
  • Molecular Typing / methods
  • Mucous Membrane / microbiology
  • Pasteurella
  • Pasteurellaceae* / classification
  • Pasteurellaceae* / genetics
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* / methods

Substances

  • DNA, Bacterial
  • RNA, Ribosomal, 16S

Supplementary concepts

  • Muribacter muris
  • Pasteurella caecimuris