Differences in cellular damage induced by dielectric barrier discharge plasma between Salmonella Typhimurium and Staphylococcus aureus

Mingming Huang; Hong Zhuang; Jianying Zhao; Jiamei Wang; Wenjing Yan; Jianhao Zhang

doi:10.1016/j.bioelechem.2019.107445

Differences in cellular damage induced by dielectric barrier discharge plasma between Salmonella Typhimurium and Staphylococcus aureus

Bioelectrochemistry. 2020 Apr:132:107445. doi: 10.1016/j.bioelechem.2019.107445. Epub 2019 Dec 18.

Authors

Mingming Huang¹, Hong Zhuang², Jianying Zhao³, Jiamei Wang⁴, Wenjing Yan³, Jianhao Zhang⁵

Affiliations

¹ College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, PR China; National Center of Meat Quality & Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
² Quality and Safety Assessment Research Unit, USDA-ARS, Athens, GA 30605, USA.
³ National Center of Meat Quality & Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
⁴ College of Food Science and Technology, Hainan University, Haikou, Hainan 570228, PR China.
⁵ National Center of Meat Quality & Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: zjhsciggyx@163.com.

PMID: 31918057
DOI: 10.1016/j.bioelechem.2019.107445

Abstract

Dielectric barrier discharge (DBD) plasma treatments are more effective against Gram negative bacteria than Gram-positive bacteria. Effects of DBD plasma were compared on Salmonella Typhimurium and Staphylococcus aureus cells. Physical cell membrane integrity and function, deoxyribonucleic acid (DNA) oxidation, and intracellular reactive oxygen species (ROS) were measured. The difference in physical damage caused by plasma on Gram-negative and Gram positive bacteria implied a difference in cellular damage pattern, which may be due to differences in cell structure and composition. Antimicrobial particles in plasma react directly with the outer membranes of Gram-negative bacteria and eventually kill them; however, ROS produced from plasma first penetrated the cell wall and membrane of Gram-positive bacteria before reacting with internal cellular components and killing the bacteria.

Keywords: Cold plasma; DNA; Membrane integrity; Nonthermal treatment; Proton motive force; ROS.

MeSH terms

Homeostasis
Microscopy, Electron, Scanning
Plasma Gases*
Reactive Oxygen Species / metabolism
Salmonella typhimurium / metabolism*
Staphylococcus aureus / metabolism*

Substances

Plasma Gases
Reactive Oxygen Species