Human lysosomal acid phosphatase: cloning, expression and chromosomal assignment

EMBO J. 1988 Aug;7(8):2343-50. doi: 10.1002/j.1460-2075.1988.tb03078.x.


A 2112-bp cDNA clone (lambda CT29) encoding the entire sequence of the human lysosomal acid phosphatase (EC was isolated from a lambda gt11 human placenta cDNA library. The cDNA hybridized with a 2.3-kb mRNA from human liver and HL-60 promyelocytes. The gene for lysosomal acid phosphatase was localized to human chromosome 11. The cDNA includes a 12-bp 5' non-coding region, an open reading frame of 1269 bp and an 831-bp 3' non-coding region with a putative polyadenylation signal 25 bp upstream of a 3' poly(A) tract. The deduced amino acid sequence reveals a putative signal sequence of 30 amino acids followed by a sequence of 393 amino acids that contains eight potential glycosylation sites and a hydrophobic region, which could function as a transmembrane domain. A 60% homology between the known 23 N-terminal amino acid residues of human prostatic acid phosphatase and the N-terminal sequence of lysosomal acid phosphatase suggests an evolutionary link between these two phosphatases. Insertion of the cDNA into the expression vector pSVL yielded a construct that encoded enzymatically active acid phosphatase in transfected monkey COS cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acid Phosphatase / genetics*
  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Blotting, Western
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11*
  • Cloning, Molecular
  • DNA / genetics
  • Female
  • Gene Expression Regulation
  • Humans
  • Lysosomes / enzymology*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Placenta / enzymology
  • Sequence Homology, Nucleic Acid
  • Software


  • DNA
  • Acid Phosphatase