Structure and function analysis of various brain subregions and pituitary in grass carp (Ctenopharyngodon idellus)

Abstract

It has been generally acknowledged that environment could alter the morphology and functional differentiation of vertebrate brain. However, as the largest group of all vertebrates, studies about the structures and functions of various brain subregions in teleost are still scarce. In this study, using grass carp as a model, histology method and RNA-sequencing were recruited to examine the microstructure and transcript levels among different brain subregions and pituitary. Histological results showed that the grass carp brain was composed of six parts, including olfactory bulb, telencephalon, hypothalamus, optic tectum, cerebellum, and medulla oblongata. In addition, compared to elasmobranchs and non-teleost bony ray-finned fishes, grass carp lost the hypothalamo-hypophyseal portal system, instead the hypophysiotropic neurons were directly terminated in the pituitary cells. At the transcriptomic level, our results suggested that the olfactory bulb might be related to reproduction and immune function. The telencephalon was deemed to be involved in the regulation of appetite and reproduction. The optic tectum might play important roles in the vision system and feeding. The hypothalamus could regulate feeding, and reproduction process. The medulla oblongata was related with the auditory system. The pituitary seemed to play pivotal roles in energy metabolism, organ development and reproduction. Finally, the correlation analysis suggested that the hypothalamus and the telencephalon were highly related, and close anatomical connection and overlapping functions suggested that the telencephalon and hypothalamus might be the regulation center of feeding and reproduction among teleost brain. This study provided a global view of the microstructures and specific functions of various brain subregions and pituitary in teleost. These results will be very helpful for further study in the neuroendocrinology regulation of growth and reproduction in teleost brain-pituitary axis.

Keywords: Hypothalamus; Olfactory bulb; Optic tectum; Pituitary; Telencephalon.