Simultaneous multi-elemental speciation of As, Hg and Pb by inductively coupled plasma mass spectrometry interfaced with high-performance liquid chromatography

Danyu Zhang; Shiwei Yang; Qingfang Ma; Jiannan Sun; Heyong Cheng; Yuanchao Wang; Jinhua Liu

doi:10.1016/j.foodchem.2019.126119

Simultaneous multi-elemental speciation of As, Hg and Pb by inductively coupled plasma mass spectrometry interfaced with high-performance liquid chromatography

Food Chem. 2020 May 30:313:126119. doi: 10.1016/j.foodchem.2019.126119. Epub 2019 Dec 30.

Authors

Danyu Zhang¹, Shiwei Yang¹, Qingfang Ma², Jiannan Sun¹, Heyong Cheng³, Yuanchao Wang⁴, Jinhua Liu⁵

Affiliations

¹ College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China.
² Key Laboratory of Organosilicon Chemistry and Material Technology, Hangzhou Normal University, Hangzhou 311121, China.
³ College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China; Key Laboratory of Organosilicon Chemistry and Material Technology, Hangzhou Normal University, Hangzhou 311121, China. Electronic address: hycheng@hznu.edu.cn.
⁴ College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China; Key Laboratory of Organosilicon Chemistry and Material Technology, Hangzhou Normal University, Hangzhou 311121, China.
⁵ Hangzhou Normal University, Qianjiang College, Hangzhou 310036, China. Electronic address: jinhua6903@163.com.

PMID: 31923869
DOI: 10.1016/j.foodchem.2019.126119

Abstract

This work establishes a hyphenated methodology coupling HPLC with ICP-MS for simultaneous speciation analysis of arsenic, mercury and lead for the first time. Four arsenicals (As(III), DMA, MMA and As(V)), four mercurials (Hg(II), MeHg, EtHg and PhHg) and three lead compounds (Pb(II), TML and TEL) were simultaneously analyzed within only 8 min with acceptable resolution (2.0-8.2 for As, 1.6-6.1 for Hg and 2.7-4.0 for Pb). The detection limits were 0.036-0.20 for As-species, 0.023-0.041 for Hg-species, and 0.0076-0.14 μg L^-1 for Pb-species. The developed method was applied for the measurement of five lotus seed samples, indicating the presence of DMA (19.6-28.2 μg kg^-1), TML (1.4-2.9 μg kg^-1), MeHg (1.2-4.8 μg kg^-1) and EtHg (0.8-2.2 μg kg^-1). This method provides a promising tool for studying the toxic, metabolic and bioavailable behaviors of arsenic, mercury and lead.

Keywords: Arsenic; HPLC-ICP-MS; Lead; Lotus seed; Mercury; Multi-elemental speciation.

MeSH terms

Arsenic / analysis*
Arsenic / chemistry
Chromatography, High Pressure Liquid
Lead / analysis*
Lead / chemistry
Limit of Detection
Mass Spectrometry*
Mercury / analysis*
Mercury / chemistry

Substances

Lead
Mercury
Arsenic