Transcriptome-wide map of m6A circRNAs identified in a rat model of hypoxia mediated pulmonary hypertension

BMC Genomics. 2020 Jan 13;21(1):39. doi: 10.1186/s12864-020-6462-y.


Background: Hypoxia mediated pulmonary hypertension (HPH) is a lethal disease and lacks effective therapy. CircRNAs play significant roles in physiological process. Recently, circRNAs are found to be m6A-modified. The abundance of circRNAs was influenced by m6A. Furthermore, the significance of m6A circRNAs has not been elucidated in HPH yet. Here we aim to investigate the transcriptome-wide map of m6A circRNAs in HPH.

Results: Differentially expressed m6A abundance was detected in lungs of HPH rats. M6A abundance in circRNAs was significantly reduced in hypoxia in vitro. M6A circRNAs were mainly from protein-coding genes spanned single exons in control and HPH groups. Moreover, m6A influenced the circRNA-miRNA-mRNA co-expression network in hypoxia. M6A circXpo6 and m6A circTmtc3 were firstly identified to be downregulated in HPH.

Conclusion: Our study firstly identified the transcriptome-wide map of m6A circRNAs in HPH. M6A can influence circRNA-miRNA-mRNA network. Furthermore, we firstly identified two HPH-associated m6A circRNAs: circXpo6 and circTmtc3. However, the clinical significance of m6A circRNAs for HPH should be further validated.

Keywords: Hypoxia mediated pulmonary hypertension; m6A circRNAs; m6A circTmtc3; m6A circXpo6.

MeSH terms

  • Animals
  • Biomarkers
  • Disease Susceptibility
  • Gene Expression Regulation
  • Hypertension, Pulmonary / etiology*
  • Hypertension, Pulmonary / metabolism*
  • Hypertension, Pulmonary / physiopathology
  • Hypoxia / genetics*
  • Hypoxia / metabolism*
  • MicroRNAs
  • RNA, Circular*
  • RNA, Messenger
  • Rats
  • Transcriptome*


  • Biomarkers
  • MicroRNAs
  • RNA, Circular
  • RNA, Messenger