Purpose: To investigate the expression of Yes-associated protein 1 (YAP1) in bladder cancer, and to study its role in regulating epithelial-mesenchymal transition in bladder cancer cells.
Material and methods: The expression of YAP1, vimentin, and E-cadherin was detected by immunohistochemistry in bladder cancer and para-carcinoma tissues. The relation between expression levels and overall survival of patients was evaluated by Kaplan-Meier estimates. Furthermore, YAP1 expression was knocked down in T24 and UMUC3 bladder cancer through transfection with YAP1-targeted small interfering RNA (siRNA), and the impact on invasiveness and epithelial-mesenchymal transition was detected.
Results: Expression levels of YAP1 were higher in bladder cancer tissues, and increased YAP1 expression significantly correlated with poor patient outcomes and poor overall survival in bladder cancer patients. Furthermore, YAP1 siRNA significantly attenuated the invasion of bladder cancer cells and could reverse their epithelial-mesenchymal transition.
Conclusion: YAP1 appears to play an important role in bladder cancer progression and is highlighted as a novel potential therapeutic target.
Keywords: Bladder cancer; e-cadherin; epithelial-mesenchymal transformation; invasion; yes-associated protein 1.
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Conflict of interest statement
Short hairpin RNA targeting FOXQ1 inhibits invasion and metastasis via the reversal of epithelial-mesenchymal transition in bladder cancer.Int J Oncol. 2013 Apr;42(4):1271-8. doi: 10.3892/ijo.2013.1807. Epub 2013 Feb 5. Int J Oncol. 2013. PMID: 23403865
Astrocyte elevated gene-1 promotes invasion and epithelial-mesenchymal transition in bladder cancer cells through activation of signal transducer and activator of transcription 3.Int J Urol. 2018 Feb;25(2):157-163. doi: 10.1111/iju.13486. Epub 2017 Nov 8. Int J Urol. 2018. PMID: 29117631
CAV-1 contributes to bladder cancer progression by inducing epithelial-to-mesenchymal transition.Urol Oncol. 2014 Aug;32(6):855-63. doi: 10.1016/j.urolonc.2014.01.005. Epub 2014 Jun 23. Urol Oncol. 2014. PMID: 24968949
GP73 promotes invasion and metastasis of bladder cancer by regulating the epithelial-mesenchymal transition through the TGF-β1/Smad2 signalling pathway.J Cell Mol Med. 2018 Mar;22(3):1650-1665. doi: 10.1111/jcmm.13442. Epub 2018 Jan 19. J Cell Mol Med. 2018. PMID: 29349903 Free PMC article.
Slug contributes to cadherin switch and malignant progression in muscle-invasive bladder cancer development.Urol Oncol. 2013 Nov;31(8):1751-60. doi: 10.1016/j.urolonc.2012.02.001. Epub 2012 Mar 14. Urol Oncol. 2013. PMID: 22421353