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, 12 (3), 1070-1077
eCollection

Yes-associated Protein 1 Promotes Bladder Cancer Invasion by Regulating Epithelial-Mesenchymal Transition

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Yes-associated Protein 1 Promotes Bladder Cancer Invasion by Regulating Epithelial-Mesenchymal Transition

Xingxing Chen et al. Int J Clin Exp Pathol.

Abstract

Purpose: To investigate the expression of Yes-associated protein 1 (YAP1) in bladder cancer, and to study its role in regulating epithelial-mesenchymal transition in bladder cancer cells.

Material and methods: The expression of YAP1, vimentin, and E-cadherin was detected by immunohistochemistry in bladder cancer and para-carcinoma tissues. The relation between expression levels and overall survival of patients was evaluated by Kaplan-Meier estimates. Furthermore, YAP1 expression was knocked down in T24 and UMUC3 bladder cancer through transfection with YAP1-targeted small interfering RNA (siRNA), and the impact on invasiveness and epithelial-mesenchymal transition was detected.

Results: Expression levels of YAP1 were higher in bladder cancer tissues, and increased YAP1 expression significantly correlated with poor patient outcomes and poor overall survival in bladder cancer patients. Furthermore, YAP1 siRNA significantly attenuated the invasion of bladder cancer cells and could reverse their epithelial-mesenchymal transition.

Conclusion: YAP1 appears to play an important role in bladder cancer progression and is highlighted as a novel potential therapeutic target.

Keywords: Bladder cancer; e-cadherin; epithelial-mesenchymal transformation; invasion; yes-associated protein 1.

Conflict of interest statement

None.

Figures

Figure 1
Figure 1
The representatives of immunohistochemical staining (A-L): YAP1 was negative expressed in para-cancerous tissues (A, B) and positive expressed in BC tissues (C, D). Vimentin was negative expressed in para-cancerous tissues (E, F) and positive expressed in BC tissues (G, H). E-cadherin was positive expressed in para-cancerous tissues (I, J) and negative expressed in BC tissues (K, L).
Figure 2
Figure 2
High YAP1 expression is related with prognosis of bladder cancer patients. Kaplan-Meier survival analysis of the overall survival time (n=55), P=0.0075.
Figure 3
Figure 3
The EMT relative mRNA and protein expression after transfection of YAP1siRNA in UMUC3 cells. *P<0.05
Figure 4
Figure 4
The EMT relative mRNA and protein expression after transfection of YAP1siRNA in T4 cells. *P<0.05.
Figure 5
Figure 5
The effect of YAP1-siRNA transfected T24 cells on the expression of Snail1 and ZEB1. *P<0.05.
Figure 6
Figure 6
Transwell experimental results. Silencing YAP1 expression inhibited the cell invasion capacity. *P<0.05.

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