Antrodia cinnamomea is an important medicinal fungus in Taiwan. This study demonstrates changes of complex sulfated polysaccharides (SPS) by fungus A. cinnamomea after ammonium sulfate-feeding and evaluates its anti-inflammatory activities. The addition of 1 mM ammonium sulfate showed maximal sulfate content of SPS in value of 1.82 mmol/g. Ammonium sulfate changes the physiochemical properties of SPS in that area percentage of SPSs (361 kDa) was increased for 1 mM ammonium sulfate to the value of 26 percentage area. SPS of 1 mM ammonium sulfate-fed A. cinnamomea (AM-SPS) had maximal inhibition of LPS-induced tumor necrosis factor (TNF-α) release in RAW264.7 macrophage. Iκ-B degradation induced by LPS in macrophages was reversed by AM-SPS. Suppression of NF-κB activation might have been responsible for the anti-inflammatory effects. Meanwhile, the inhibition was also due to suppressing the AKT, and ERK signaling pathway. Our finding suggests that ammonium sulfate is a useful nutrient for production of SPS for neutraceutical and pharmaceutical applications.
Keywords: Ammonium sulfate; Anti-inflammatory activity; Antrodia cinnamomea; Sulfated polysaccharides.
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