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, 11 (5), 2605-2612
eCollection

Serum miR-126-3p Level Is Down-Regulated in Sepsis Patients

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Serum miR-126-3p Level Is Down-Regulated in Sepsis Patients

Chao Chen et al. Int J Clin Exp Pathol.

Abstract

Background: Endothelial injury is part of the pathogenesis of sepsis. The microRNA-126 (miR-126) was previously identified as an endothelial biomarker and is known to play a critical role in preserving endothelial cell integrity. However, the role of miRNA-126 in sepsis is unclear.

Method: Blood samples were collected from sepsis patients at the first Affiliated Hospital of Sun Yat-sen University within 24 h (n = 60) and on day 7 (n = 51) after diagnosis, and once from control subjects (n = 46). MiR-126-3p expression was evaluated by quantitative real-time PCR. The miR-126-3p level was correlated with clinical data and a set of routine and experimental biomarkers. The outcome of sepsis patients was determined by follow-up at 28 days after collection of blood samples on day 7.

Result: MiR-126-3p level was significantly downregulated in sepsis patients 24 h after diagnosis compared with control subjects. Degree of downregulation of serum miR-126-3p correlated with the severity of sepsis. To determine the diagnostic accuracy of miR-126-3p, the receiver operating characteristic (ROC) was performed and the AUC of miR-126-3p was 0.735. Furthermore, serum miR-126-3p concentration at this time point was correlated with the expression markers of systemic inflammation, bacterial infection, and renal and hepatic dysfunction. However, serum miR-126-3p level on day 7 day did not differ between surviving sepsis patients and those who died.

Conclusion: These results indicate that miR-126-3p could be a diagnostic biomarker for sepsis.

Keywords: Sepsis; diagnosis; endothelial injury; miR-126-3p; prognosis.

Conflict of interest statement

None.

Figures

Figure 1
Figure 1
Patient enrollment and blood sample collection.
Figure 2
Figure 2
Serum miR-126-3p levels in pediatric sepsis patients and controls. A. Serum miR-126-3p level in sepsis patients (n = 60) and control subjects (n = 46) were significantly different on the first day after diagnosis. B. MiR-126-3p level on the first day was down-regulated in pediatric patients with sepsis as compared to those without sepsis. C. MiR-126-3p levels in patients without sepsis (n = 25) were similar to those in healthy controls (n = 21). D. The serum miR-126-3p level was associated with the degree of sepsis. E. Serum miR-126-3p level of sepsis patients with PCIS scores ≥80 was significantly higher than those with scores <80. F. Serum miR-126-3p levels did not vary according to the source of sepsis.
Figure 3
Figure 3
ROC curve analysis of the diagnostic accuracy of serum miR-126-3p levels.
Figure 4
Figure 4
A. MiR-126-3p level of sepsis patients on day 7 was significantly higher than on day 1. B. MiR-126-3p level on day 7 in sepsis patients did not differ between patients who survived and those who had died after a 28 day follow-up visit.

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