Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes

Nature. 1988 Nov 24;336(6197):348-52. doi: 10.1038/336348a0.


Gene targeting--homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences--in mouse embryo-derived stem cells promises to provide a means to generate mice of any desired genotype. We describe a positive nd negative selection procedure that enriches 2,000-fold for those cells that contain a targeted mutation. The procedure was applied to the isolation of hprt- and int-2- mutants, but it should be applicable to any gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Clone Cells
  • Mice
  • Mice, Transgenic*
  • Mutation*
  • Proto-Oncogenes*
  • Restriction Mapping
  • Stem Cells
  • Transfection*