Sequencing proteins from acrylamide gels

T E Kennedy; K Wager-Smith; A Barzilai; E R Kandel; J D Sweatt

doi:10.1038/336499a0

Sequencing proteins from acrylamide gels

Nature. 1988 Dec 1;336(6198):499-500. doi: 10.1038/336499a0.

Authors

T E Kennedy¹, K Wager-Smith, A Barzilai, E R Kandel, J D Sweatt

Affiliation

¹ Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, New York 10032.

PMID: 3194033
DOI: 10.1038/336499a0

Abstract

The analytical power of high-resolution two-dimensional gel electrophoresis has been coupled with molecular cloning techniques to allow the sequencing of proteins directly from preparative gels.

MeSH terms

Amino Acid Sequence*
Animals
Aplysia / analysis
Cloning, Molecular*
DNA / genetics
DNA Probes
Electrophoresis, Polyacrylamide Gel*
Molecular Sequence Data
Peptide Fragments / isolation & purification
Proteins* / genetics
Proteins* / isolation & purification
Proteins* / metabolism
Serine Endopeptidases / metabolism

Substances

DNA Probes
Peptide Fragments
Proteins
DNA
Serine Endopeptidases
glutamyl endopeptidase