Background: Previous studies have shown that ASPM is a tumorigenesis associated protein, mutations in ASPM can lead to MCPH. This study mainly explores the relationship between the ASPM expression of lung adenocarcinoma and the development and prognosis of lung cancer. The aim of this study was to investigate the relationship between the expression of abnormal spindle-like microcephaly-associated protein (ASPM) in lung adenocarcinoma and the development and prognosis in lung cancer.
Methods: A total of 90 cases of lung adenocarcinoma tissue specimens and 90 cases of benign pulmonary lesions were collected, the expression of ASPM was detected by immunohistochemical technique, and the expression of ASPM in 12 pairs of tissues was detected by real-time quantity polymerase chain reaction and western blot.
Results: (1) The expression of ASPM in the tissue specimens of benign pulmonary lesions was negative while the expression level of ASPM in lung adenocarcinoma was significantly higher than lung tissue of benign pulmonary lesions (P<0.05). (2) The expression level of ASPM has no remarkable difference in lung adenocarcinoma with lymph node development and the lung cancer without lymph node development, there was no statistically significant (P<0.05). (3) The ASPM expression level in the tumor's size ≥4 cm was significantly higher than than of the size<4 cm (P<0.05). (4) Hierarchical analysis results show that T stage is related to ASPM expression level (P<0.05). (5) The high expression level of ASPM in lung adenocarcinoma was significantly positively correlated with the poor prognosis (P<0.05).
Conclusions: (1) The expression level of ASPM in lung cancer is obviously increased and closely related to the progress of lung adenocarcinoma. (2) The expression level of ASPM in lung adenocarcinoma was significantly positively correlated with poor prognosis (P<0.05). (3) Detection of the expression level of ASPM in lung adenocarcinoma help to predict the prognosis of lung adenocarcinoma in advance.
【中文题目：ASPM在肺腺癌中的表达及其与肺腺癌的进展和预后关系的研究】 【中文摘要：背景与目的 已有的研究表明人类异常纺锤体样小头畸形相关蛋白（abnormal spindle-likemicrocephaly-associated protein, ASPM）是一个与肿瘤发生发展相关的蛋白，ASPM的突变会导致常染色体隐性原发性小头畸形。本研究主要是探讨肺腺癌组织中的表达情况与肺癌进展和预后的关系。方法 收集90例肺腺癌组织标本和对应的90例肺良性病变组织标本，应用免疫组织化学技术检测其中90对组织中ASPM的表达情况，应用实时荧光定量核酸扩增法和蛋白印迹法的方法检测其中12对组织中ASPM的表达情况。结果 肺良性病变组织标本中ASPM表达为阴性，肺腺癌组织中ASPM的表达为阳性结果且表达水平均显著高于肺良性病变肺组织（P<0.05）。伴有淋巴结进展的肺腺癌中ASPM的表达水平与不伴有淋巴结进展的肺癌没有差异，无统计学意义（P>0.05）。肿瘤大小≥4 cm的ASPM表达水平均显著高于肿瘤大小<4 cm（P<0.05）。分层分析结果表明T分期与ASPM表达层度相关（P<0.05）。肺腺癌组织中ASPM的高表达水平与不良预后呈正相关（P<0.05）。结论 肺腺癌中ASPM的表达水平明显升高，且与肺腺癌的进展有密切关系。肺腺癌组织中ASPM的表达水平与不良预后呈正相关（P<0.05）。检测肺腺癌中ASPM的表达水平有助于提早的预测肺腺癌的预后。】 【中文关键词：肺腺癌；人类异常纺锤体样小头畸形相关蛋白；免疫组织化学；预后】.
Keywords: ASPM; Immunohistochemical; Lung adenocarcinoma; Prognosis.