Long noncoding RNA SNHG14 promotes osteosarcoma progression via miR-433-3p/FBXO22 axis

Biochem Biophys Res Commun. 2020 Mar 12;523(3):766-772. doi: 10.1016/j.bbrc.2020.01.016. Epub 2020 Jan 14.


Long noncoding RNA small nucleolus RNA host gene 14 (SNHG14) has been shown to exert oncogenic functions in seceral cancers, but its role in osteosarcoma still unclear. In this present study, we found that SNHG14 was significantly upregulated in osteosarcoma tissues and cell lines. Knockdown SNHG14 expression significantly inhibited osteosarcoma cell proliferation through inducing apoptosis. Further functional assays revealed that SNHG14 knockdown dramatically suppressed cell migration and invasion. Bioinformatics analysis and luciferase assays identified that microRNA-433-3p (miR-433-3p) was a direct target of SNHG14, and directly targeted F-box only protein 22 (FBXO22). Mechanistic analysis demonstrated that SNHG14 acted as a ceRNA in modulating osteosarcoma proliferation, migration and invasion by decoying miR-433-3p to upregulate FBXO22 expression. We also observed that knockdown of FBXO22 and SNHG14 and overexpression of miR-433-3p both dramatically suppressed osteosarcoma cell proliferation, migration and invasion, but induced cell apoptosis. Moreover, the suppresive effect of SNHG14 knockdown on osteosarcoma cell proliferation, invasion and migration was attenuated by miR-433-3p inhibitor. Our findings demonstrated that SNHG14 promoted osteosarcoma progression by acting as a ceRNA for miR-433-3p to regulate FBXO22 expression, suggesting that SNHG14 may serve as a potential therapeutic target in osteosarcoma patients.

Keywords: Invasion; Migration; Osteosarcoma; Proliferation; SNHG14; miR-433–3p/FBXO22.

MeSH terms

  • Bone Neoplasms / genetics*
  • Bone Neoplasms / pathology
  • Cell Line, Tumor
  • Cell Proliferation
  • Disease Progression
  • F-Box Proteins / genetics*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MicroRNAs / genetics*
  • Neoplasm Invasiveness / genetics
  • Neoplasm Invasiveness / pathology
  • Osteosarcoma / genetics*
  • Osteosarcoma / pathology
  • RNA, Long Noncoding / genetics*
  • Receptors, Cytoplasmic and Nuclear / genetics*


  • F-Box Proteins
  • FBXO22 protein, human
  • MIRN433 microRNA, human
  • MicroRNAs
  • RNA, Long Noncoding
  • Receptors, Cytoplasmic and Nuclear