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. 2020 Jan;84(1):67-73.

Antioxidative enzyme activity and total antioxidant capacity in serum of dogs with degenerative mitral valve disease

Marcin Michałek  1 , Aleksandra Tabiś  1 , Alicja Cepiel  1 , Agnieszka Noszczyk-Nowak  1
Affiliations

Antioxidative enzyme activity and total antioxidant capacity in serum of dogs with degenerative mitral valve disease

Marcin Michałek et al. Can J Vet Res. 2020 Jan.

Abstract
in English, French

This study was designed to evaluate the antioxidative status of serum by measuring its total antioxidant capacity, as well as the antioxidant enzyme activity (superoxide dismutase, catalase, and glutathione reductase), in dogs with various stages of degenerative mitral valve disease (DMVD) compared to healthy controls. In total, 71 client-owned dogs in different stages of DMVD, which included healthy controls, took part in the study. Following an anamnesis, clinical examination, standard transthoracic echocardiograpic examination, chest X-ray, complete blood (cell) count, and serum biochemistry, dogs were divided into 2 study groups. Blood was drawn from each dog once at the time of presentation and selected antioxidant parameters were measured using commercially available assay kits. The activity of superoxide dismutase gradually decreased in the more advanced stages of DMVD, while the activity of catalase was significantly higher in the group of dogs with asymptomatic DMVD compared to healthy controls and dogs with symptomatic DMVD. No significant changes were noted in total antioxidant capacity and the activity of glutathione reductase. Results suggested that DMVD has a significant impact on the activity of superoxide dismutase and catalase in the serum of the tested dogs. Knowledge of changes in the activity of antioxidative enzymes may warrant further studies, possibly to evaluate the potential role of compounds with antioxidative properties in the clinical outcome of dogs with DMVD.

La présente étude a été conçue afin d’évaluer le statut antioxydant du sérum en mesurant sa capacité antioxydante totale, ainsi que l’activité antioxydante enzymatique (superoxyde dismutase, catalase, et glutathion réductase), chez des chiens avec des degrés divers de maladie dégénérative de la valvule mitrale (DMVD) comparativement à des témoins en santé. Au total, 71 chiens appartenant à des clients à différents stades de DMVD, qui incluaient des témoins en santé, ont pris part à cette étude. À la suite de la prise d’anamnèse, d’un examen clinique, d’un examen échocardiographie transthoracique standard, de radiographie thoracique, d’un comptage cellulaire sanguin complet, et d’analyse biochimique sérique, les chiens étaient séparés en deux groupes d’étude. Du sang fut prélevé de chaque chien une fois au moment de la présentation et les paramètres antioxydants sélectionnés furent mesurés à l’aide d’une trousse disponible commercialement. L’activité de la superoxyde dismutase diminuait graduellement dans les stades plus avancés de DMVD, alors que l’activité de la catalase était significativement plus élevée dans le groupe de chiens avec une DMVD asymptomatique comparativement aux témoins en santé et aux chiens avec une DMVD symptomatique. Aucun changement significatif n’était noté dans la capacité antioxydante totale et dans l’activité de la glutathion réductase. Les résultats suggèrent que la DMVD a un impact significatif sur l’activité de la superoxyde dismutase, et de la catalase dans le sérum des chiens testés. Des connaissances sur les changements dans l’activité des enzymes antioxydantes pourraient justifier des études additionnelles, possiblement pour évaluer le rôle potentiel de produits avec des propriétés antioxydantes dans le devenir clinique de chiens avec DMVD.(Traduit par Docteur Serge Messier).

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Figures

Figure 1
Figure 1
The superoxide dismutase (SOD) activity of serum in healthy dogs and dogs with degenerative mitral valve disease (DMVD). * P < 0.05; ** P < 0.01. A — healthy controls; B — asymptomatic DMVD; C — symptomatic, stable DMVD.
Figure 2
Figure 2
The catalase (CAT) activity of serum in healthy dogs and dogs with degenerative mitral valve disease (DMVD). ** P < 0.01. A — healthy controls; B — asymptomatic DMVD; C — symptomatic, stable DMVD.
Figure 3
Figure 3
The glutathione reductase (GR) activity of serum in healthy dogs and dogs with degenerative mitral valve disease (DMVD). A — healthy controls; B — asymptomatic DMVD; C — symptomatic, stable DMVD.
Figure 4
Figure 4
The total antioxidant capacity (TAC) of serum in healthy dogs and dogs with degenerative mitral valve disease (DMVD). A — healthy controls; B — asymptomatic DMVD; C — symptomatic, stable DMVD.
See this image and copyright information in PMC

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