Clitoria ternatea L. petal bioactive compounds display antioxidant, antihemolytic and antihypertensive effects, inhibit α-amylase and α-glucosidase activities and reduce human LDL cholesterol and DNA induced oxidation

Graziela Bragueto Escher; Mariza Boscacci Marques; Mariana Araújo Vieira do Carmo; Luciana Azevedo; Marianna Miranda Furtado; Anderson S Sant'Ana; Marcia Cristina da Silva; Maria Inês Genovese; Mingchun Wen; Liang Zhang; Won Young Oh; Fereidoon Shahidi; Neiva Deliberali Rosso; Daniel Granato

doi:10.1016/j.foodres.2019.108763

Clitoria ternatea L. petal bioactive compounds display antioxidant, antihemolytic and antihypertensive effects, inhibit α-amylase and α-glucosidase activities and reduce human LDL cholesterol and DNA induced oxidation

Food Res Int. 2020 Feb:128:108763. doi: 10.1016/j.foodres.2019.108763. Epub 2019 Oct 31.

Authors

Affiliations

¹ Food Science and Technology Graduate Program, State University of Ponta Grossa, 84030-900 Ponta Grossa, PR, Brazil. Electronic address: grazibragueto@hotmail.com.
² Department of Chemistry, State University of Ponta Grossa, 84030-900 Ponta Grossa, PR, Brazil.
³ Deprtment of Biological Sciences, Federal University of Alfenas, 37130-000 Alfenas, MG, Brazil.
⁴ Department of Food Science, Faculty of Food Engineering, University of Campinas, 13083-862 Campinas, SP, Brazil.
⁵ Department of Food, Federal Institute of Education, Science and Technology of Rio de Janeiro, 20270-021 Rio de Janeiro, RJ, Brazil.
⁶ Department of Food and Experimental Nutrition, Laboratory of Bioactive Compounds, University of São Paulo, 05508-900 São Paulo, Brazil.
⁷ State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 230036 Hefei, PR China.
⁸ Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada.
⁹ Food Science and Technology Graduate Program, State University of Ponta Grossa, 84030-900 Ponta Grossa, PR, Brazil.
¹⁰ Food Science and Technology Graduate Program, State University of Ponta Grossa, 84030-900 Ponta Grossa, PR, Brazil; Innovative Food System, Production Systems Unit - Natural Resources Institute Finland (LUKE) - Open Innovation House, FI-02150 Espoo, Finland. Electronic address: daniel.granato@luke.fi.

PMID: 31955736
DOI: 10.1016/j.foodres.2019.108763

Abstract

The purpose of this study was to use a statistical approach to optimise the experimental conditions regarding the extraction of bioactive compounds, and to analyse the in vitro functional properties of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of Clitoria ternatea petals. The results showed that the factors of temperature and time influenced the extraction of phenolic compounds, antioxidant activity and the physicochemical parameters. Simultaneous optimisation showed that the same levels of bioactive compounds were extracted when using temperatures from 11.7 to 68.3 °C and times from 8.47 to 51.12 min. Principal component analysis revealed the experimental conditions that provided the extraction producing the highest level of phenolic content (40 °C/30 min). The CLE showed antimicrobial activity; protective effect against hemolysis of erythrocytes; inhibition of α-amylase, α-glucosidase and angiotensin-I-converting (ACE-I) enzymes; and inhibition of lipid peroxidation. The CLE and PPE demonstrated oxygen radical absorption capacity; inhibition of DNA strand scission; inhibition of LDL cholesterol oxidation; intracellular antioxidant activity against reactive oxygen species (>100 μg/mL); and no cytotoxicity (IC₅₀, GI₅₀ and LC₅₀ > 900 μg/mL) against A549, HCT8 and IMR90 cell lines.

Keywords: 2,4-Dihydroxybenzoic acid (PubChem CID: 1491); Anthocyanins; Antihypertensive activity; Caffeic acid (PubChem CID: 689043); Delphinidin-3-O-glucoside (PubChem CID: 443650); Gallic acid (PubChem CID: 370); Inhibitor of DNA strand scission; Principal component analysis; Procyanidin A2 (PubChem CID: 124025); Protocatechuic acid (PubChem CID: 72); Quercetin-3-rutinoside (PubChem CID: 5280805); Response surface methodology; Syringic acid (PubChem CID: 10742); Unconventional foods; p-Coumaric acid (PubChem CID: 637542).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antihypertensive Agents / metabolism*
Antioxidants / metabolism*
Cholesterol, LDL / drug effects*
Clitoria / metabolism*
DNA
Flowers
Hemolysis / drug effects*
Humans
In Vitro Techniques
Oxidation-Reduction / drug effects
Plant Extracts / metabolism
Principal Component Analysis
alpha-Amylases / metabolism*
alpha-Glucosidases / metabolism*

Substances

Antihypertensive Agents
Antioxidants
Cholesterol, LDL
Plant Extracts
DNA
alpha-Amylases
alpha-Glucosidases