MAP7 promotes migration and invasion and progression of human cervical cancer through modulating the autophagy

Li Zhang; Xudong Liu; Lina Song; Hui Zhai; Chaohua Chang

doi:10.1186/s12935-020-1095-4

MAP7 promotes migration and invasion and progression of human cervical cancer through modulating the autophagy

Cancer Cell Int. 2020 Jan 13:20:17. doi: 10.1186/s12935-020-1095-4. eCollection 2020.

Authors

Li Zhang^#¹, Xudong Liu^#², Lina Song¹, Hui Zhai¹, Chaohua Chang³

Affiliations

¹ 1Department of Gynaecology and Obstetrics, Jinan Women and Children Health Hospital, No. 2 Jianguo Xiaojing 3rd Road Center Area, Jinan, Shandong 250001 China.
² 2Department of Pain, Qilu Hospital of Shandong University, Jinan, China.
³ 3Department of Anethesia, Jinan Women and Children Health Hospital, No. 2 Jianguo Xiaojing 3rd Road Center Area, Jinan, Shandong 250001 China.

^# Contributed equally.

Abstract

Background: Microtubule-associated proteins 7(MAP7) was reported to be engaged into the function of neuronal function. The function of MAP7 in human cervical cancer (CC) was unknown. We aimed to uncover the function and mechanism of MAP7 on CC.

Methods: We applied qRT-PCR, western blot and immunochemistry to detect the expression difference between normal tissue and CC. In vitro, we establish MAP7 stable knocking down and overexpression cell lines and investigated the function and underlying mechanism of MAP7 in CC.

Results: Both mRNA and protein of MAP7 were upregulated in CC compared with the normal tissue. MAP7 was correlated with the clinical stage and tumor size and lymph node metastasis. MAP7 promotes the invasion and migration of CC cell lines. We next detected EMT pathway and autophagy associated pathway. MAP7 promotes the EMT through modulating the autophagy.

Conclusion: Taken above, our results showed that MAP7 promotes the migration and invasion and EMT through modulating the autophagy.

Keywords: Cervical cancer; Epithelial-mesenchymal transition; MAP7; Migration and invasion.