Effect of interactions between various humic acid fractions and iron nanoparticles on the toxicity to white rot fungus

Abstract

Humic acid plays an important role in controlling the toxicity of nanoparticles to organisms. However, little is known about the influence of different fractions of dissolved humic acid (DHA) from soil on the toxicity of nanoparticles to organisms. The concentration of γ-Fe₂O₃ and the exposure time affected the malondialdehyde (MDA) content, reactive oxygen species (ROS) production and lactate dehydrogenase (LDH) activity in P. chrysosporium cells and were inversely proportional to the relative activities of the cells. P. chrysosporium was exposed to γ-Fe₂O₃ and DHA₁ for 3 h, 6 h and 12 h. Catalase (CAT) and peroxidase (POD) activities were generally higher than control. Particularly, under the influence of 50 mg/L DHA₁ and different concentrations of γ-Fe₂O₃ (10 and 50 mg/L), the CAT and POD activities were higher than those of cells exposed to γ-Fe₂O₃ alone. Conversely, both activities of P. chrysosporium exposed to DHA₄ combined with γ-Fe₂O₃ for 12 h were lower than those of cells exposed to γ-Fe₂O₃ alone and gradually decreased with increasing DHA₄ concentration (0, 10 and 50 mg/L). The μ-XAFS normalized spectrum indicated that Fe³⁺ entering the cells tended to transform into Fe²⁺ as the stress time prolonged. TEM analysis confirmed the toxicity of high concentrations of γ-Fe₂O₃ to P. chrysosporium. The comet assay showed that DHA₄ in soil enhanced the toxicity of γ-Fe₂O₃ to P. chrysosporium more than DHA₁ did. Namely, compared to DHA₁, DHA₄ made it easier for nano-Fe₂O₃ to enter P. chrysosporium cells, causing more toxicity of γ-Fe₂O₃ to P. chrysosporium.

Keywords: Comet assay; Different fractions of dissolved humic acid; Iron oxide nanomaterials; P. chrysosporium; TEM; μ-XAFS.