A novel oligosaccharide isolated from Hericium erinaceus and its protection against LPS-induced Caco-2 cells via the TLR4/NF-κB pathway

J Food Biochem. 2020 Mar;44(3):e13135. doi: 10.1111/jfbc.13135. Epub 2020 Jan 20.

Abstract

A novel oligosaccharide showed that protection against LPS-induced Caco-2 cells was purified from the mycelium of Hericium erinaceus (HE). WEP-1 is mainly composed of neutral monosaccharides with molecular weight of 4,010 Da and of mannose, glucose, and galactose in a molar ratio of 1.2:16.9:1. The structure of WEP-1 includes α-D-Glc (1 → 3) and β-D-Gal (1 → 3) as the backbone with β-D-Glc (1 → 3) as branches attached to the C-4 position and β-D-Man as a terminal residue. The oligosaccharide reduced acetic acid-induced colonic mucosa injury in rats. It also showed significant protection against LPS-induced Caco-2 cells via the TLR4/NF-κB pathway. PRACTICAL APPLICATIONS: In the study, the oligosaccharide from HE has the potential to be developed into functional foods or medicines for the treatment of intestinal diseases. The protection against LPS-induced Caco-2 cells via the TLR4/NF-κB pathway may be a key target for the pharmacological activity of HE.

Keywords: Hericium erinaceus; Caco-2; NF-κB; oligosaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caco-2 Cells
  • Hericium
  • Humans
  • Lipopolysaccharides / toxicity
  • NF-kappa B*
  • Oligosaccharides / pharmacology
  • Rats
  • Toll-Like Receptor 4*

Substances

  • Lipopolysaccharides
  • NF-kappa B
  • Oligosaccharides
  • TLR4 protein, human
  • Tlr4 protein, rat
  • Toll-Like Receptor 4