Golgi organization is regulated by proteasomal degradation

Nat Commun. 2020 Jan 21;11(1):409. doi: 10.1038/s41467-019-14038-9.


The Golgi is a dynamic organelle whose correct assembly is crucial for cellular homeostasis. Perturbations in Golgi structure are associated with numerous disorders from neurodegeneration to cancer. However, whether and how dispersal of the Golgi apparatus is actively regulated under stress, and the consequences of Golgi dispersal, remain unknown. Here we demonstrate that 26S proteasomes are associated with the cytosolic surface of Golgi membranes to facilitate Golgi Apparatus-Related Degradation (GARD) and degradation of GM130 in response to Golgi stress. The degradation of GM130 is dependent on p97/VCP and 26S proteasomes, and required for Golgi dispersal. Finally, we show that perturbation of Golgi homeostasis induces cell death of multiple myeloma in vitro and in vivo, offering a therapeutic strategy for this malignancy. Taken together, this work reveals a mechanism of Golgi-localized proteasomal degradation, providing a functional link between proteostasis control and Golgi architecture, which may be critical in various secretion-related pathologies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Autoantigens / metabolism
  • Cell Line, Tumor / transplantation
  • Disease Models, Animal
  • Golgi Apparatus / drug effects
  • Golgi Apparatus / metabolism*
  • HEK293 Cells
  • Humans
  • Intracellular Membranes / metabolism
  • Ionophores / pharmacology
  • Ionophores / therapeutic use*
  • Membrane Proteins / metabolism
  • Mice
  • Monensin / pharmacology
  • Monensin / therapeutic use
  • Multiple Myeloma / drug therapy*
  • Multiple Myeloma / pathology
  • Proteasome Endopeptidase Complex / metabolism*
  • Proteolysis / drug effects
  • Proteostasis / drug effects
  • Proteostasis / physiology*
  • Ubiquitination / drug effects
  • Valosin Containing Protein / metabolism


  • Autoantigens
  • Golgin subfamily A member 2
  • Ionophores
  • Membrane Proteins
  • Monensin
  • Proteasome Endopeptidase Complex
  • ATP dependent 26S protease
  • Valosin Containing Protein