Osteosarcoma cells induce differentiation of mesenchymal stem cells into cancer associated fibroblasts through Notch and Akt signaling pathway

Yu-Ming Wang; Wei Wang; En-Duo Qiu

Osteosarcoma cells induce differentiation of mesenchymal stem cells into cancer associated fibroblasts through Notch and Akt signaling pathway

Int J Clin Exp Pathol. 2017 Aug 1;10(8):8479-8486. eCollection 2017.

Authors

Yu-Ming Wang¹, Wei Wang¹, En-Duo Qiu¹

Affiliation

¹ Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Insititute China.

PMID: 31966700
PMCID: PMC6965449

Abstract

Cancer-associated fibroblasts plays an important role in tumor progession, growth and metastasis. However, the mechanisms of mesenchymal stem cells differentiation to cancer-associated fibroblasts induced by tumor cells remains unclear. This study aimed to explore whether osteosarcoma cells can also induce BMSCs into CAFs through cells co-culture system, and identify the probably signailing pathway that mediates differentiation of BMSCs to CAFs. The results showed that osteosarcoma cells can induce BMSCs to exhibit the typical characteristics of CAFs, with increased expression of α-SMA, the most reliable marker for CAFs. Morever, we also reveals that Notch signaling acts upstream of Akt signaling in HBMSCs-TAFs differentiation.

Keywords: Mesenchymal stem cell; akt signaling pathway; cancer-associated fibroblasts; notch signaling pathway; osteosarcoma cell.