Development of a lateral flow aptamer assay strip for facile identification of theranostic exosomes isolated from human lung carcinoma cells

Anal Biochem. 2020 Apr 1:594:113591. doi: 10.1016/j.ab.2020.113591. Epub 2020 Jan 20.

Abstract

Exosomes are Extracellular Vesicles (EV) that own unique structural features and functions and have gradually become the hot research spot in recent years. The tumor-derived exosomes contain various types of useful biological information, and medical identification of exosomes relied on the specific characterization of membrane surface proteins. In this study, in order to rapidly identify non-small cell lung cancer (NSCLC)-derived exosomes, based on an aptamer against CD63 protein on exosome membrane, a low cost lateral flow aptamer assay (LFAA) test strip using nanogold particles as visualization probes was successfully developed for facile identification of A549 exosomes isolated from human lung carcinoma cells diluted from 6.4 × 109 particles/mL herein.

Keywords: Aptamer; Exosomes; Lateral flow aptamer assay (LFAA); Non-small cell lung cancer (NSCLC).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • A549 Cells
  • Aptamers, Nucleotide / chemistry*
  • Biological Assay / methods*
  • Carcinoma, Non-Small-Cell Lung / chemistry
  • Exosomes / chemistry*
  • Gold / chemistry
  • Humans
  • Lung Neoplasms / chemistry
  • Metal Nanoparticles / chemistry
  • Tetraspanin 30 / chemistry*
  • Theranostic Nanomedicine / methods*

Substances

  • Aptamers, Nucleotide
  • CD63 protein, human
  • Tetraspanin 30
  • Gold