Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 367 (6476), 405-411

Single-cell Transcriptional Diversity Is a Hallmark of Developmental Potential

Affiliations

Single-cell Transcriptional Diversity Is a Hallmark of Developmental Potential

Gunsagar S Gulati et al. Science.

Abstract

Single-cell RNA sequencing (scRNA-seq) is a powerful approach for reconstructing cellular differentiation trajectories. However, inferring both the state and direction of differentiation is challenging. Here, we demonstrate a simple, yet robust, determinant of developmental potential-the number of expressed genes per cell-and leverage this measure of transcriptional diversity to develop a computational framework (CytoTRACE) for predicting differentiation states from scRNA-seq data. When applied to diverse tissue types and organisms, CytoTRACE outperformed previous methods and nearly 19,000 annotated gene sets for resolving 52 experimentally determined developmental trajectories. Additionally, it facilitated the identification of quiescent stem cells and revealed genes that contribute to breast tumorigenesis. This study thus establishes a key RNA-based feature of developmental potential and a platform for delineation of cellular hierarchies.

Similar articles

See all similar articles

Publication types

LinkOut - more resources

Feedback