Whole genome sequence of an edible and medicinal mushroom, Hericium erinaceus (Basidiomycota, Fungi)

Abstract

Hericium erinaceus is a well-known culinary and medicinal mushroom in China. The biological and genetic studies on this mushroom is rare, thereby hindering the breeding of elite cultivars. Herein, we performed de novo sequencing and assembly of H. erinaceus monokaryon CS-4 genome using the Illumina and PacBio platform. The generated genome was 41.2 Mb in size with a N50 scaffold size of 3.2 Mb, and encoded 10,620 putative predicted genes. A wide spectrum of carbohydrate-active enzymes, with the total number of 341 CAZymes, involved in lignocellulose degradation were identified in H. erinaceus. A total of 447 transcription factors were identified. This present study also characterized genome-wide microsatellites and developed markers in H. erinaceus. A comprehensive microsatellite markers database (HeSSRDb) containing the information of 904 markers was generated. These genomic resources and newly-designed molecular markers would enrich the toolbox for biological and genetic studies in H. erinaceus.

Keywords: CAZymes; Hericium erinaceus; Microsatellite markers database; Transcription factors; Whole genome sequencing.