Transcriptional coactivator with PDZ-binding motif stimulates epidermal regeneration via induction of amphiregulin expression after ultraviolet damage

Biochem Biophys Res Commun. 2020 Mar 26;524(1):242-248. doi: 10.1016/j.bbrc.2020.01.079. Epub 2020 Jan 23.


Ultraviolet (UV) irradiation induces the proliferation and differentiation of keratinocytes in the basal layer of the epidermis, which increases epidermal thickness in skin regeneration. However, the mechanism underlying this phenomenon is not yet known in detail. In this study, we aimed to demonstrate that the transcriptional coactivator with PDZ-binding motif (TAZ) stimulates epidermal regeneration by increasing keratinocyte proliferation. During epidermal regeneration, TAZ is localized in the nucleus of keratinocytes of the basal layer and stimulates epidermal growth factor receptor (EGFR) signaling. TAZ depletion in keratinocytes decreased EGFR signaling activation, which delays epidermal regeneration. Interestingly, TAZ stimulated the transcription of amphiregulin (AREG), a ligand of EGFR, through TEAD-mediated transcriptional activation. Together, these results show that TAZ stimulates EGFR signaling through AREG induction, suggesting that it plays an important role in epidermal regeneration.

Keywords: AREG; EGFR signal; Skin regeneration; UV damage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amphiregulin / genetics*
  • Amphiregulin / metabolism
  • Animals
  • Cell Proliferation / radiation effects
  • Epidermis / physiology*
  • Epidermis / radiation effects
  • ErbB Receptors / metabolism
  • Gene Deletion
  • Humans
  • Keratinocytes / metabolism
  • Keratinocytes / radiation effects
  • Ligands
  • Male
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Regeneration* / radiation effects
  • Signal Transduction / radiation effects
  • Trans-Activators / metabolism*
  • Transcription, Genetic* / radiation effects
  • Ultraviolet Rays*


  • Adaptor Proteins, Signal Transducing
  • Amphiregulin
  • Ligands
  • Trans-Activators
  • Wwtr1 protein, mouse
  • ErbB Receptors