Distribution of carbapenem resistant Acinetobacter baumannii with blaADC-30 and induction of ADC-30 in response to beta-lactam antibiotics

Birson Ingti; Supriya Upadhyay; Monalisha Hazarika; Annie Bakorlin Khyriem; Deepjyoti Paul; Prithwis Bhattacharya; S R Joshi; Debajyoti Bora; Debadatta Dhar; Amitabha Bhattacharjee

doi:10.1016/j.resmic.2020.01.002

Distribution of carbapenem resistant Acinetobacter baumannii with bla_ADC-30 and induction of ADC-30 in response to beta-lactam antibiotics

Res Microbiol. 2020 Apr-Jun;171(3-4):128-133. doi: 10.1016/j.resmic.2020.01.002. Epub 2020 Jan 25.

Affiliations

¹ Department of Microbiology, Assam University, Silchar, 788011, India; Department of Microbiology, Royal Global University, Guwahati, 781035, India.
² Department of Biotechnology & Bioinformatics, North Eastern Hill University, Shillong, 793022, Meghalaya, India.
³ Department of Microbiology, Assam University, Silchar, 788011, India.
⁴ Department of Microbiology, North Eastern Indira Gandhi Regional Institute of Health and Medical Sciences, Shillong, Meghalaya, India.
⁵ Department of Anesthesiology, North Eastern Indira Gandhi Regional Institute of Health and Medical Sciences, Shillong, Meghalaya, India.
⁶ Department of Biotechnology & Bioinformatics, North Eastern Hill University, Shillong, 793022, Meghalaya, India. Electronic address: srjoshi2006@yahoo.co.in.
⁷ Department of Statistics, Dibrugarh University, Dibrugarh, India.
⁸ Department of Microbiology, Silchar Medical College and Hospital, Silchar, India.

PMID: 31988011
DOI: 10.1016/j.resmic.2020.01.002

Abstract

A wide range of intrinsic Acinetobacter-derived cephalosporinases (ADC) along with other carbapenemases has now been detected in Acinetobacter baumannii leaving clinicians with few treatment options. The present study reports the spread of ADC-30 co-producing KPC-2 along with other β-lactamases among carbapenem resistant A. baumannii strains obtained from ICU patients in two Indian hospitals. Primer extension analysis revealed higher transcript level of the ADC gene when induced with cefoxitin at 8 μg/ml (170 fold), ceftriaxone at 8 μg/ml (136 fold), ceftazidime at 4 μg/ml (65 fold), cefepime at 8 μg/ml (77 fold) and aztreonam at 8 μg/ml (21 fold) when compared with the basal level without antibiotic pressure. Slight increase in expression of bla_ADC-30 when induced with imipenem and meropenem at 0.25 μg/ml (3 and 6 fold) was observed and may help in conferring resistance to carbapenem. MLST analysis revealed the circulation of A. baumannii sequence types ST188, ST386, ST583 and ST390 in these hospitals.

Keywords: Acinetobacter baumannii; Carbapenem resistant; bla(ADC-30).

MeSH terms

Acinetobacter baumannii / classification
Acinetobacter baumannii / drug effects*
Acinetobacter baumannii / genetics*
Anti-Bacterial Agents / pharmacology*
Carbapenems / pharmacology*
Cephalosporinase / genetics*
Drug Resistance, Multiple, Bacterial
Gene Expression Regulation, Bacterial
Microbial Sensitivity Tests
Multilocus Sequence Typing
Sequence Analysis, DNA
beta-Lactam Resistance*

Substances

Anti-Bacterial Agents
Carbapenems
Cephalosporinase