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Detection of 2019 Novel Coronavirus (2019-nCoV) by Real-Time RT-PCR


Detection of 2019 Novel Coronavirus (2019-nCoV) by Real-Time RT-PCR

Victor M Corman et al. Euro Surveill.


Background: The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur.

Aim: We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available.

Methods: Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.

Results: The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive - Global (EVAg), a European Union infrastructure project.

Conclusion: The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.

Keywords: 2019-nCoV; RT-PCR; Wuhan; diagnostics; laboratory; novel coronavirus; outbreak; testing.

Conflict of interest statement

Conflict of interest: None declared.


Figure 1
Figure 1
Relative positions of amplicon targets on the SARS coronavirus and the 2019 novel coronavirus genome
Figure 2
Figure 2
Partial alignments of oligonucleotide binding regions, SARS-related coronaviruses (n = 9)
Figure 3
Figure 3
Determination of limits of detection based on SARS coronavirus genomic RNA and 2019 novel coronavirus-specific in vitro transcribed RNA

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    1. World Health Organization (WHO). Coronavirus. Geneva: WHO; 2020 [Accessed 21 Jan 2020]. Available from:
    1. Zhang Y-Z. Novel 2019 coronavirus genome. Virological. [Accessed 21 Jan 2020]. Available from:
    1. de Groot RJ, Baker SC, Baric R, Enjuanes L, Gorbalenya AE, Holmes KV, et al. Family Coronaviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ. Virus taxonomy: classification and nomenclature of viruses: ninth report of the International Committee on Taxonomy of Viruses. London; Waltham: Academic Press; 2012. p. 806-20.
    1. Peiris JS, Yuen KY, Osterhaus AD, Stöhr K. The severe acute respiratory syndrome. N Engl J Med. 2003;349(25):2431-41. 10.1056/NEJMra032498 - DOI - PubMed
    1. World Health Organization. (WHO. Novel Coronavirus (2019-nCoV). Situation report – 1. Geneva: WHO; 21 Jan 2020. Available from:

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