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Serum IgG Anti- Toxoplasma gondii Antibody Concentrations Do Not Correlate Nested PCR Results in Blood Donors


Serum IgG Anti- Toxoplasma gondii Antibody Concentrations Do Not Correlate Nested PCR Results in Blood Donors

Fabiana Nakashima et al. Front Cell Infect Microbiol.


Background: Toxoplasma gondii infects millions of individuals worldwide. This protozoan is food and water-borne transmitted but blood transfusion and organ transplantation constitute alternative forms for transmission. However, the influence of IgG anti-T. gondii antibodies in molecular analysis carried out in peripheral blood still remain unclear. This study aimed to investigate the serum IgG anti-T. gondii antibody concentrations correlate Nested PCR results in blood donors. Methods: 750 blood donors were enrolled. IgM and IgG anti-T. gondii antibodies were assessed by ELISA (DiaSorin, Italy). Nested PCR was performed with primers JW62/JW63 (288 bp) and B22/B23 (115 bp) of the T. gondii B1 gene. The mean values of IgG concentration were compared for PCR positive and PCR Negative blood donors using the t-test or Mann-Whitney according to the normal distribution (p-value ≤ 0.05). Results: 361 (48.1%) blood donors presented positive serology as follow: IgM+/IgG-: 5 (0.6%); IgM+/IgG+: 21 (2.8%); IgM-/IgG+: 335 (44.7%) and 389 (51.9%), negative serology. From 353 blood donors with positive serology tested, the Nested PCR was positive in 38 (10.8%) and negative in 315 (89.2%). There were no differences statistically significant between the mean values of serum IgG anti-T. gondii antibody concentrations and the Nested PCR results. Conclusions: In conclusion, our data show that variations in the serum IgG anti-T. gondii antibody concentrations do not correlate T. gondii parasitemia detected by Nested PCR in chronically infected healthy blood donors.

Keywords: Nested PCR; Toxoplasma gondii; blood donation and transfusion; blood donnors; molecular diagnosis; serology; serology assay; transfusion.


Figure 1
Figure 1
Electrophoretic profile of fragments of B1 gene from T. gondii genomic DNA extracted from peripheral blood of blood donors, amplified by Nested PCR. In (A), the first amplification is showing a fragment of 288 base pairs; in (B), the second amplification is showing a fragment of 115 base pairs. M, molecular marker; PC, Positive Control; CN, Negative Control.

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    1. Botein E. F., Darwish A., El-Tantawy N. L., EL-baz R., Eid M. I., Shaltot A. M., et al. . (2019). Serological and molecular screening of umbilical cord blood for Toxoplasma gondii infection. Transpl. Infect. Dis. 21:e13117. 10.1111/tid.13117 - DOI - PubMed
    1. Brenier-Pinchart M. P., Capderou E., Bertini R. L., Bailly S., Fricker-Hidalgo H., Varlet-Marie E., et al. . (2015). Molecular diagnosis of toxoplasmosis: value of the buffy coat for the detection of circulating Toxoplasma gondii. Diagn. Microbiol. Infect. Dis. 82, 289–291. 10.1016/j.diagmicrobio.2015.04.004 - DOI - PubMed
    1. Camilo L. M., Pereira-Chioccola V. L., Gava R., Meira-Strejevitch C. D. S., Vidal J. E., Brandão de Mattos C. C., et al. . (2017). Molecular diagnosis of symptomatic toxoplasmosis: a 9-year retrospective and prospective study in a referral laboratory in São Paulo, Brazil. Braz. J. Infect. Dis. 21, 638–647. 10.1016/j.bjid.2017.07.003 - DOI - PubMed
    1. Cohen S. B., Denkers E. Y. (2015). The gut mucosal immune response to Toxoplasma gondii. Parasite Immunol. 37, 108–117. 10.1111/pim.12164 - DOI - PubMed
    1. Coombes J. L., Hunter C. A. (2015). Immunity to Toxoplasma gondii–into the 21st century. Parasite Immunol. 37, 105–107. 10.1111/pim.12177 - DOI - PubMed

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