Background: Plant NADPH oxidase (NOX), also known as respiratory burst oxidase homolog (rboh), encoded by the rboh gene, is a key enzyme in the reactive oxygen species (ROS) metabolic network. It catalyzes the formation of the superoxide anion (O2•-), a type of ROS. In recent years, various studies had shown that members of the plant rboh gene family were involved in plant growth and developmental processes as well as in biotic and abiotic stress responses, but little is known about its functional role in upland cotton.
Results: In the present study, 26 putative Ghrboh genes were identified and characterized. They were phylogenetically classified into six subfamilies and distributed at different densities across 18 of the 26 chromosomes or scaffolds. Their exon-intron structures, conserved domains, synteny and collinearity, gene family evolution, regulation mediated by cis-acting elements and microRNAs (miRNAs) were predicted and analyzed. Additionally, expression profiles of Ghrboh gene family were analyzed in different tissues/organs and at different developmental stages and under different abiotic stresses, using RNA-Seq data and real-time PCR. These profiling studies indicated that the Ghrboh genes exhibited temporal and spatial specificity with respect to expression, and might play important roles in cotton development and in stress tolerance through modulating NOX-dependent ROS induction and other signaling pathways.
Conclusions: This comprehensive analysis of the characteristics of the Ghrboh gene family determined features such as sequence, synteny and collinearity, phylogenetic and evolutionary relationship, expression patterns, and cis-element- and miRNA-mediated regulation of gene expression. Our results will provide valuable information to help with further gene cloning, evolutionary analysis, and biological function analysis of cotton rbohs.
Keywords: Expression patterns; Gene family; Rboh; Reactive oxygen species; Upland cotton.
Conflict of interest statement
The authors declare no conflict of interest.
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Genome-wide mining of respiratory burst homologs and its expression in response to biotic and abiotic stresses in Triticum aestivum.Genes Genomics. 2019 Sep;41(9):1027-1043. doi: 10.1007/s13258-019-00821-x. Epub 2019 May 28. Genes Genomics. 2019. PMID: 31140145
Genome-wide identification and expression analysis of NADPH oxidase genes in response to ABA and abiotic stresses, and in fibre formation in Gossypium.PeerJ. 2020 Jan 17;8:e8404. doi: 10.7717/peerj.8404. eCollection 2020. PeerJ. 2020. PMID: 31988810 Free PMC article.
Legume NADPH Oxidases Have Crucial Roles at Different Stages of Nodulation.Int J Mol Sci. 2016 May 18;17(5):680. doi: 10.3390/ijms17050680. Int J Mol Sci. 2016. PMID: 27213330 Free PMC article. Review.
Plant signaling networks involving Ca(2+) and Rboh/Nox-mediated ROS production under salinity stress.Front Plant Sci. 2015 Jun 10;6:427. doi: 10.3389/fpls.2015.00427. eCollection 2015. Front Plant Sci. 2015. PMID: 26113854 Free PMC article. Review.