3D-printed graphene direct electron transfer enzyme biosensors

Adaris M López Marzo; Carmen C Mayorga-Martinez; Martin Pumera

doi:10.1016/j.bios.2019.111980

3D-printed graphene direct electron transfer enzyme biosensors

Biosens Bioelectron. 2020 Mar 1:151:111980. doi: 10.1016/j.bios.2019.111980. Epub 2019 Dec 23.

Authors

Adaris M López Marzo¹, Carmen C Mayorga-Martinez¹, Martin Pumera²

Affiliations

¹ Center for Advanced Functional Nanorobots, Department of Inorganic Chemistry, University of Chemistry and Technology Prague, Technická 5, 166 28, Prague 6, Czech Republic.
² Center for Advanced Functional Nanorobots, Department of Inorganic Chemistry, University of Chemistry and Technology Prague, Technická 5, 166 28, Prague 6, Czech Republic; Department of Chemical and Biomolecular Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea; Department of Medical Research, China Medical University Hospital, China Medical University, No. 91 Hsueh-Shih Road, Taichung, Taiwan; Future Energy and Innovation Laboratory, Central European Institute of Technology, Brno University of Technology, Purkyňova 656/123, Brno, CZ-616 00, Czech Republic. Electronic address: pumera.research@gmail.com.

PMID: 31999587
DOI: 10.1016/j.bios.2019.111980

Abstract

Three-dimensional (3D) printing technology offers attractive possibilities for many fields. In electrochemistry, 3D printing technology has been used to fabricate customized 3D-printed electrodes as a platform to develop bio/sensing, energy generation and storage devices. Here, we use a 3D-printed graphene/polylactic (PLA) electrode made by additive manufacturing technology and immobilize horseradish peroxidase (HRP) to create a direct electron transfer enzyme-based biosensors for hydrogen peroxide detection. Gold nanoparticles are included in the system to confirm and facilitate heterogeneous electron transfer. This work opens a new direction for the fabrication of third-generation electrochemical biosensors using 3D printing technology, with implications for applications in the environmental and biomedical fields.

Keywords: 3D-printed electrodes; Direct electron transfer; Graphene/polylactic acid; Horseradish peroxidase.

MeSH terms

Biosensing Techniques*
Electron Transport / genetics
Enzymes / chemistry
Enzymes / genetics
Enzymes / isolation & purification*
Graphite / chemistry*
Printing, Three-Dimensional*

Substances

Enzymes
Graphite