Germline development in rat revealed by visualization and deletion of Prdm14

Development. 2020 Feb 21;147(4):dev183798. doi: 10.1242/dev.183798.

Abstract

Primordial germ cells (PGCs), the founder cells of the germline, are specified in pre-gastrulating embryos in mammals, and subsequently migrate towards gonads to mature into functional gametes. Here, we investigated PGC development in rats, by genetically modifying Prdm14, a unique marker and an essential PGC transcriptional regulator. We trace PGC development in rats, for the first time, from specification until the sex determination stage in fetal gonads using Prdm14 H2BVenus knock-in rats. We uncover that the crucial role of Prdm14 in PGC specification is conserved between rat and mice, by analyzing Prdm14-deficient rat embryos. Notably, loss of Prdm14 completely abrogates the PGC program, as demonstrated by failure of the maintenance and/or activation of germ cell markers and pluripotency genes. Finally, we profile the transcriptome of the post-implantation epiblast and all PGC stages in rat to reveal enrichment of distinct gene sets at each transition point, thereby providing an accurate transcriptional timeline for rat PGC development. Thus, the novel genetically modified rats and data sets obtained in this study will advance our knowledge on conserved versus species-specific features for germline development in mammals.

Keywords: Prdm14; Primordial germ cell; Rat.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Crosses, Genetic
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / physiology
  • Female
  • Gastrula / physiology
  • Gene Deletion
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Germ Cells / cytology*
  • Heterozygote
  • Male
  • Mice
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / physiology
  • Rats
  • Sex Determination Processes
  • Transcription Factors / genetics*
  • Transcription Factors / physiology
  • Transcription, Genetic

Substances

  • DNA-Binding Proteins
  • Prdm14 protein, mouse
  • Prdm14 protein, rat
  • RNA-Binding Proteins
  • Transcription Factors