Enhanced protein thermostability from designed mutations that interact with alpha-helix dipoles

Nature. 1988 Dec 15;336(6200):651-6. doi: 10.1038/336651a0.

Abstract

Two different genetically engineered amino-acid substitutions designed to interact with alpha-helix dipoles in T4 lysozyme are shown to increase the thermal stability of the protein. Crystallographic analyses of the mutant lysozyme structures suggest that the stabilization is due to electrostatic interaction and does not require precise hydrogen bonding between the substituted amino acid and the end of the alpha-helix.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aspartic Acid / metabolism*
  • Binding Sites
  • Chemical Phenomena
  • Chemistry
  • Crystallization
  • DNA-Binding Proteins / metabolism*
  • Genetic Engineering / methods
  • Hydrogen-Ion Concentration
  • Muramidase / genetics
  • Muramidase / metabolism*
  • Mutation
  • Structure-Activity Relationship
  • T-Phages / enzymology
  • Temperature
  • Thermodynamics

Substances

  • DNA-Binding Proteins
  • Aspartic Acid
  • Muramidase